Yoo Dae Young, Jung Hyo Young, Kwon Hyun Jung, Kim Jong Whi, Nam Sung Min, Chung Jin Young, Choi Jung Hoon, Kim Dae Won, Yoon Yeo Sung, Hwang In Koo
1Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, Seoul, 08826 Korea.
2Department of Biochemistry and Molecular Biology, Research Institute of Oral Sciences, College of Dentistry, Gangneung-Wonju National University, Gangneung, Gangwon, 25457 Korea.
Food Sci Biotechnol. 2016 Dec 31;25(6):1761-1766. doi: 10.1007/s10068-016-0268-3. eCollection 2016.
In this experiment, we verified the effects of Léveille stem extract (DMS) on hypothyroidism-induced oxidative stress in the hippocampus of rats. Hypothyroidism was induced in rats by treating them with 0.03% 2-mercapto-1-methyl-imidazole dissolved in drinking water for 5 weeks. DMS (100 mg/kg) was also orally administered to the rats during the same period and the animals were sacrificed at 12 weeks of age. DMS administration tended to ameliorate these hypothyroidism-induced changes in serum triiodothyronine (T), thyroxine (T), and thyroid-stimulating hormone levels. DMS administration significantly reduced the hypothyroidism-induced increases in reactive oxygen species production as well as in lipid peroxidation in the hippocampus. In addition, DMS administration increased hippocampal Cu, Zn-superoxide dismutase (SOD1), catalase (CAT), and glutathione peroxidase (GPx) levels. These results suggest that DMS potentially ameliorates hypothyroidism-induced neuroendocrine phenotypes and oxidative stress in the hippocampus via the induction of antioxidant enzymes.
在本实验中,我们验证了勒韦耶茎提取物(DMS)对大鼠海马体中甲状腺功能减退诱导的氧化应激的影响。通过给大鼠饮用溶解有0.03% 2-巯基-1-甲基咪唑的水5周来诱导大鼠甲状腺功能减退。在此期间,也给大鼠口服DMS(100毫克/千克),并在大鼠12周龄时将其处死。给予DMS倾向于改善甲状腺功能减退引起的血清三碘甲状腺原氨酸(T)、甲状腺素(T)和促甲状腺激素水平的变化。给予DMS显著降低了甲状腺功能减退诱导的海马体中活性氧生成以及脂质过氧化的增加。此外,给予DMS增加了海马体中铜锌超氧化物歧化酶(SOD1)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GPx)的水平。这些结果表明,DMS可能通过诱导抗氧化酶来改善甲状腺功能减退诱导的海马体神经内分泌表型和氧化应激。