交让木提取物对汞暴露大鼠海马的抗氧化作用
Antioxidant effects of Dendropanax morbifera Léveille extract in the hippocampus of mercury-exposed rats.
作者信息
Kim Woosuk, Kim Dae Won, Yoo Dae Young, Jung Hyo Young, Kim Jong Whi, Kim Dong-Woo, Choi Jung Hoon, Moon Seung Myung, Yoon Yeo Sung, Hwang In Koo
机构信息
Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, Seoul, 151-742, South Korea.
Department of Biochemistry and Molecular Biology, Research Institute of Oral Sciences, College of Dentistry, Kangneung-Wonju National University, Gangneung, 210-702, South Korea.
出版信息
BMC Complement Altern Med. 2015 Jul 23;15:247. doi: 10.1186/s12906-015-0786-1.
BACKGROUND
Dendropanax morbifera Léveille has been employed for the treatment of infectious diseases using folk medicine. In this study, we evaluated the antioxidant effects of a leaf extract of Dendropanax morbifera Léveille in the hippocampus of mercury-exposed rats.
METHODS
Seven-week-old Sprague-Dawley rats received a daily intraperitoneal injection of 5 μg/kg dimethylmercury and/or oral Dendropanax morbifera Léveille leaf extract (100 mg/kg) for 4 weeks. Animals were sacrificed 2 h after the last dimethylmercury and/or leaf extract treatment. Mercury levels were measured in homogenates of hippocampal tissue, a brain region that is vulnerable to mercury toxicity. In addition, we measured reactive oxygen species production, lipid peroxidation levels, and antioxidant levels in these hippocampal homogenates.
RESULTS
Treatment with Dendropanax morbifera Léveille leaf extract significantly reduced mercury levels in hippocampal homogenates and attenuated the dimethylmercury-induced increase in the production of reactive oxygen species and formation of malondialdehyde. In addition, this leaf extract treatment significantly reversed the dimethylmercury-induced reduction in the hippocampal activities of Cu, Zn-superoxide dismutase, catalase, glutathione peroxidase, and glutathione-S-transferase.
CONCLUSION
These results suggest that a leaf extract of Dendropanax morbifera Léveille had strong antioxidant effects in the hippocampus of mercury-exposed rats.
背景
辽东楤木已被用于民间医学治疗传染病。在本研究中，我们评估了辽东楤木叶提取物对汞暴露大鼠海马体的抗氧化作用。
方法
7周龄的Sprague-Dawley大鼠每天腹腔注射5μg/kg二甲基汞和/或口服辽东楤木叶提取物（100mg/kg），持续4周。在最后一次二甲基汞和/或叶提取物处理后2小时处死动物。在海马组织（一个易受汞毒性影响的脑区）的匀浆中测量汞含量。此外，我们还测量了这些海马匀浆中的活性氧生成、脂质过氧化水平和抗氧化剂水平。
结果
辽东楤木叶提取物处理显著降低了海马匀浆中的汞含量，并减弱了二甲基汞诱导的活性氧生成增加和丙二醛形成。此外，这种叶提取物处理显著逆转了二甲基汞诱导的海马中铜锌超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶和谷胱甘肽-S-转移酶活性的降低。
结论
这些结果表明，辽东楤木叶提取物对汞暴露大鼠的海马体具有强大的抗氧化作用。
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