从蓝蟹和虾壳中提取壳聚糖作为大豆溶质絮凝剂的新途径。
New route of chitosan extraction from blue crabs and shrimp shells as flocculants on soybean solutes.
作者信息
Lee Yongjae, Kim Hyun-Wook, Brad Kim Yuan H
机构信息
1Department of Nutrition and Food Science, Texas A&M University, 2476 TAMU, College Station, TX 77843 USA.
2Department of Animal Science and Biotechnology, Gyeongnam National University of Science and Technology, Jinju, 52725 South Korea.
出版信息
Food Sci Biotechnol. 2017 Dec 12;27(2):461-466. doi: 10.1007/s10068-017-0270-4. eCollection 2018 Apr.
The chitosan extracted from blue crabs and shrimp shells using calcium oxide (deproteinization) followed by deacetylation which eliminated the demineralization step to reduce the chemical usage and environmental protection. The extracted chitosan examined the flocculation to soybean solutes. The optical density (OD), solid%, and purity% (carbohydrates/soluble solids) after flocculation were measured. The OD was significantly decreased from 0.76 to 0.16 with blue crabs and 0.06 with shrimp shells chitosan-acetate dosing (0.5 g/L). The removal of about 68 and 66% solids was achieved by the addition of 0.5 g/L chitosan-acetate. The purity% was reached about 80% with blue crabs, and 78% with shrimp shells chitosan-acetate. The results of this study verified that the calcium oxide treatment should remove protein and increase the chitin extraction yield on blue crab and shrimp shells. This new route of chitosan extraction should be a useful method for making flocculants in the soybean solutes.
采用氧化钙（脱蛋白）从蓝蟹和虾壳中提取壳聚糖，随后进行脱乙酰化，省去了脱矿质步骤，以减少化学物质的使用并保护环境。对提取的壳聚糖进行了大豆溶质絮凝试验。测量了絮凝后的光密度（OD）、固含量和纯度（碳水化合物/可溶性固体）。使用蓝蟹壳聚糖 - 醋酸盐（0.5 g/L）投加量时，OD从0.76显著降至0.16，使用虾壳壳聚糖 - 醋酸盐时降至0.06。添加0.5 g/L壳聚糖 - 醋酸盐可去除约68%和66%的固体。蓝蟹壳聚糖 - 醋酸盐的纯度约为80%，虾壳壳聚糖 - 醋酸盐的纯度约为78%。本研究结果证实，氧化钙处理应能去除蛋白质并提高蓝蟹和虾壳中几丁质的提取率。这种新的壳聚糖提取途径应是制备大豆溶质絮凝剂的一种有用方法。
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