Taser Behiye, Ozkan Hakan, Adiguzel Ahmet, Orak Tugba, Baltaci Mustafa Ozkan, Taskin Mesut
Department of Molecular Biology and Genetics, Faculty of Science and Letters, Agri Ibrahim Cecen University, Agri, Turkey.
Department of Molecular Biology and Genetics, Science Faculty, Ataturk University, Erzurum, Turkey.
Int J Biol Macromol. 2021 Jul 31;183:1191-1199. doi: 10.1016/j.ijbiomac.2021.05.062. Epub 2021 May 11.
In this study, chitin extraction from shrimp shell powder (SSP) using locally isolated Paenibacillus jamilae BAT1 (GenBank: MN176658), the preparation of chitosan from the extracted chitin, and the characterization and biological activity (antimicrobial and antioxidant) of the prepared chitosan (PC) were investigated. It was determined that P. jamilae BAT1 did not have chitinase activity but showed high protease activity and protein removal potential. Optimum pH, shell concentration and incubation time for deproteinization were determined as 7.0, 60 g/L and 4 days, respectively. Addition of KHPO or MgSO did not affect chitin extraction and deproteinization yield. The maximum yields of deproteinization, demineralization and chitin extraction yields were 87.67, 41.95 and 24.5%, respectively. The viscosity-average molecular weight of PC was determined as 1.41 × 10 g/mol. The deacetylation degree of PC (86%) was found to be higher that of commercial chitosan (CC) (78%). DPPH scavenging activity of PC (IC 0.59 mg/mL) was higher than that of CC (IC 3.72 mg/mL). PC was found to have higher antimicrobial activity against the bacteria E. coli and S. aureus and the yeast C. albicans when compared to CC. This is the first study on the use of the bacterium P. jamilae in biological chitin extraction.
在本研究中，对使用本地分离的詹氏芽孢杆菌BAT1（GenBank：MN176658）从虾壳粉（SSP）中提取几丁质、从提取的几丁质制备壳聚糖以及对制备的壳聚糖（PC）进行表征和生物活性（抗菌和抗氧化）研究。结果表明，詹氏芽孢杆菌BAT1没有几丁质酶活性，但具有较高的蛋白酶活性和蛋白质去除潜力。确定脱蛋白的最适pH、壳浓度和孵育时间分别为7.0、60 g/L和4天。添加KHPO或MgSO不影响几丁质提取和脱蛋白产率。脱蛋白、脱矿质和几丁质提取的最大产率分别为87.67%、41.95%和24.5%。PC的粘均分子量测定为1.41×10 g/mol。发现PC的脱乙酰度（86%）高于商业壳聚糖（CC）（78%）。PC的DPPH清除活性（IC 0.59 mg/mL）高于CC（IC 3.72 mg/mL）。与CC相比，发现PC对大肠杆菌、金黄色葡萄球菌和白色念珠菌具有更高的抗菌活性。这是关于使用詹氏芽孢杆菌进行生物几丁质提取的首次研究。
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