Tappaz M L, Kakucska I, Paut L, Makara G B
Brain Res Bull. 1986 Nov;17(5):711-6. doi: 10.1016/0361-9230(86)90205-4.
The effects of hypothalamic cuts at various rostro-caudal levels on the GABAergic innervation of the neurointermediate lobe of the pituitary gland have been studied. The GABAergic innervation was visualized through glutamate-decarboxylase (GAD) immunocytochemistry. Caudal hypothalamic cuts which transected the pituitary stalk completely abolished the GAD immunoreactive plexus. Rostral cuts which separated about one-third of the median eminence and arcuate nucleus from the pituitary gland decreased the GAD-immunoreactive network in the intermediate lobe but did not affect the neural lobe significantly. Although the precise location of the cell bodies giving rise to the GABAergic innervation of the neurointermediate lobe remains unknown, our findings indicate that their projections are descending ones. They are severed by rostral hypothalamic cuts and show a rostrocaudal arrangement. It is likely that the GABAergic endings of the intermediate lobe originate in the rostral hypothalamus, probably in the rostral part of the arcuate nucleus and/or in the anterior periventricular area. The GABAergic fibers in the neural lobe have a more caudal origin than those innervating the intermediate lobe.
研究了下丘脑不同 rostro - 尾侧水平切断对垂体神经中间叶GABA能神经支配的影响。通过谷氨酸脱羧酶（GAD）免疫细胞化学观察GABA能神经支配。完全横断垂体柄的尾侧下丘脑切断术完全消除了GAD免疫反应性神经丛。将约三分之一的正中隆起和弓状核与垂体分离的头侧切断术减少了中间叶的GAD免疫反应性网络，但对神经叶没有显著影响。尽管产生神经中间叶GABA能神经支配的细胞体的确切位置尚不清楚，但我们的研究结果表明它们的投射是下行的。它们被头侧下丘脑切断术切断，并呈头 - 尾排列。中间叶的GABA能末梢可能起源于头侧下丘脑，可能在弓状核的头侧部分和/或室周前区。神经叶中的GABA能纤维比支配中间叶的纤维起源更靠尾侧。
