Department of Life Science and Medical Bioscience, Graduate School of Advanced Science and Engineering, Waseda University, Tokyo, 162-8480, Japan.
Department of Plastic surgery, National Defense Medical College, Saitama, 359-8513, Japan.
J Biomed Mater Res B Appl Biomater. 2019 Jul;107(5):1363-1371. doi: 10.1002/jbm.b.34228. Epub 2018 Sep 28.
Stem cell transplantation is expected to be an effective early-phase treatment for deep burn injuries and intractable ulcers. Localizing and proliferating stem cells on the lesion utilizing engineered scaffolds is important for this treatment. In this study, we demonstrated in situ transplantation of adipose-tissue derived stem cells (ASCs) organized on free-standing porous polymer ultrathin films (referred to as "porous nanosheets") to a skin defect model in diabetic mice. Porous nanosheets were prepared by a combination of micro-gravure coating with macrophase separation of poly(d,l-lactic acid) and polystyrene under a roll-to-roll process and solvent etching process with cyclohexane. The permeable structure of porous nanosheets (thickness of 150 nm, average pore diameter of 4 μm) allowed for proliferation of ASCs and also provided sufficient nutrient inflow into multilayered ASC constructs. Then, transplantation of a trilayered ASC-laden porous nanosheet achieved homogeneous transference of ASCs onto the skin lesion. Transplanted ASCs contributed to wound healing in a dorsal skin defect model in diabetic mice. Thus, cell transplantation using porous nanosheets will be a new method for promoting wound healing in diabetic and other kinds of refractory ulcers. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1363-1371, 2019.
干细胞移植有望成为治疗深度烧伤和难治性溃疡的有效早期治疗方法。利用工程支架在病变部位定位和增殖干细胞对于这种治疗方法非常重要。在这项研究中，我们展示了将组织在独立式多孔聚合物超薄膜（称为“多孔纳米片”）上的脂肪组织来源干细胞（ASCs）原位移植到糖尿病小鼠的皮肤缺损模型中。多孔纳米片是通过微凹版涂布与聚（DL-乳酸）和聚苯乙烯的大相差分离相结合，在辊到辊工艺和环己烷的溶剂蚀刻工艺下制备的。多孔纳米片的可渗透结构（厚度为 150nm，平均孔径为 4μm）允许 ASCs 增殖，并为多层 ASC 构建体提供足够的营养流入。然后，移植三层载有 ASC 的多孔纳米片可实现 ASC 均匀转移到皮肤损伤部位。移植的 ASCs 有助于糖尿病小鼠背部皮肤缺损模型中的伤口愈合。因此，使用多孔纳米片进行细胞移植将成为促进糖尿病和其他类型难治性溃疡愈合的新方法。©2018 Wiley Periodicals, Inc. J 生物材料研究杂志 B：应用生物材料 107B：1363-1371，2019。
