Instituto Dante Pazzanese de Cardiologia, São Paulo, Brazil.
Universidade Federal do ABC, São Bernardo do Campo, São Paulo, Brazil.
J Biomed Mater Res B Appl Biomater. 2019 Jul;107(5):1462-1470. doi: 10.1002/jbm.b.34238. Epub 2018 Sep 28.
Fibrous scaffolds have become popular in tissue engineering (TE) due to their morphological resemblance to extracellular matrix components. While electrospinning is the most common technique in the field, solution blow spinning is an emerging technique with great potential. One of its many advantages is that it can produce aligned fibers with a very simple experimental setup. This work aimed to fabricate poly(ε-caprolactone) mats with aligned fibers and compare them to nonaligned ones. For that, samples were produced using three rotational speeds of a cylindrical collector and characterized in terms of fiber alignment and diameter, mechanical properties, wettability, and biological response. Results showed that with a static collector, fibers were randomly deposited and nonaligned. As the speed was increased, the fibers began to align (as proven by image analysis), resulting in a change in mechanical behavior, but no differences in fiber diameter. Cells cultured on aligned samples were more elongated, and a higher alignment degree seemed to favor cellular growth. The results confirmed the potential of this up-and-coming technique to produce aligned fibers for TE. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1462-1470, 2019.
纤维支架由于其形态与细胞外基质成分相似，在组织工程（TE）中变得非常流行。虽然静电纺丝是该领域最常见的技术，但溶液吹纺丝是一种具有巨大潜力的新兴技术。其众多优点之一是，它可以用非常简单的实验装置生产出取向纤维。本工作旨在制备具有取向纤维的聚（ε-己内酯）（PCL）垫，并将其与无规纤维进行比较。为此，使用圆柱形收集器的三种旋转速度来制备样品，并从纤维取向和直径、机械性能、润湿性和生物响应等方面进行了表征。结果表明，在使用静态收集器时，纤维是随机沉积的，没有取向。随着速度的增加，纤维开始取向（通过图像分析证明），导致机械性能发生变化，但纤维直径没有差异。在取向样品上培养的细胞更加细长，较高的取向度似乎有利于细胞生长。结果证实了这项新兴技术在生产用于 TE 的取向纤维方面的潜力。© 2018 Wiley Periodicals, Inc. J 生物材料研究杂志 B：应用生物材料 107B：1462-1470，2019。
