College of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066004, Hebei, People's Republic of China.
College of Animal Science and Technology, Agricultural University of Hebei Province, Baoding 071001, Hebei, People's Republic of China; Qinhuangdao Animal Disease Control Center, Qinhuangdao 066001, Hebei, People's Republic of China.
Genomics. 2019 Dec;111(6):1395-1403. doi: 10.1016/j.ygeno.2018.09.012. Epub 2018 Sep 27.
Bashang long-tail chickens are an indigenous breed with dual purpose in China (meat and eggs) but have low egg laying performance. To improve the low egg laying performance, a genome-wide analysis of mRNAs and long noncoding RNAs (lncRNAs) from Bashang long-tail chickens and Hy-Line brown layers was performed. A total of 16,354 mRNAs and 8691 lncRNAs were obtained from ovarian follicles. Between the breeds, 160 mRNAs and 550 lncRNAs were found to be significantly differentially expressed. Integrated network analysis suggested some differentially expressed genes were involved in ovarian follicular development through oocyte meiosis, progesterone-mediated oocyte maturation, and cell cycle. The impact of lncRNAs on cis and trans target genes, indicating some lncRNAs may play important roles in ovarian follicular development. The current results provided a catalog of chicken ovarian follicular lncRNAs and genes for further study to understand their roles in regulation of egg laying performance.
坝上长尾鸡是中国的一种兼用型本土鸡种（肉用和蛋用），但产蛋性能较低。为了提高低产蛋性能，对坝上长尾鸡和海兰褐蛋鸡的卵巢卵泡进行了 mRNA 和长链非编码 RNA（lncRNA）的全基因组分析。从卵巢卵泡中获得了 16354 个 mRNAs 和 8691 个 lncRNAs。在两个品种之间，发现 160 个 mRNAs 和 550 个 lncRNAs 存在显著差异表达。综合网络分析表明，一些差异表达基因通过卵母细胞减数分裂、孕激素介导的卵母细胞成熟和细胞周期参与卵巢卵泡发育。lncRNAs 对顺式和反式靶基因的影响表明，一些 lncRNAs 可能在卵巢卵泡发育中发挥重要作用。目前的研究结果为进一步研究鸡卵巢卵泡 lncRNAs 和基因在调节产蛋性能中的作用提供了一个目录。
