School of Food Science and Engineering , South China University of Technology , Guangzhou 510640 , China.
Guangdong Food Green Processing and Nutrition Regulation Technologies Research Center , Guangzhou 510650 , China.
J Agric Food Chem. 2018 Oct 17;66(41):10760-10768. doi: 10.1021/acs.jafc.8b04676. Epub 2018 Oct 8.
This study aimed to investigate the protective effects of bovine elastin hydrolysates on UV-induced skin photoaging in mice and to identify the potent antiphotoaging peptides. Results showed that the ingestion of elastin peptides could obviously ameliorate epidermis hyperplasia and fibroblast apoptosis, and increase the content of hydroxyproline and water in photoaging skin in vivo ( p < 0.05). Furthermore, four peptides with elastase inhibitory activity were purified and identified, including GLPY, PY, GLGPGVG, and GPGGVGAL. Interestingly, GLPY and GPGGVGAL exhibited the highest inhibition activity with 58.77% and 42.91% at 10 mΜ, respectively. This might be attributed to the N-terminal Gly, C-terminal Leu, and Pro at the third position of the N-terminus, which showed stronger affinity and interaction with elastase. Moreover, GLPY and GPGGVGAL could also inhibit the apoptosis of fibroblasts effectively at 50 μΜ ( p < 0.01). It suggested that elastin peptides had great potential to prevent and regulate skin photoaging.
本研究旨在探讨牛弹性蛋白水解物对紫外线诱导的小鼠皮肤光老化的保护作用，并鉴定有效的抗光老化肽。结果表明，弹性蛋白肽的摄入可明显改善表皮增生和成纤维细胞凋亡，并增加体内光老化皮肤中羟脯氨酸和水的含量（p<0.05）。此外，还纯化和鉴定了四种具有弹性蛋白酶抑制活性的肽，包括 GLPY、PY、GLGPGVG 和 GPGGVGAL。有趣的是，GLPY 和 GPGGVGAL 分别在 10 mM 时表现出最高的抑制活性，为 58.77%和 42.91%。这可能归因于 N 端 Gly、C 端 Leu 和 N 端第三位 Pro，它们与弹性蛋白酶表现出更强的亲和力和相互作用。此外，GLPY 和 GPGGVGAL 还能有效抑制 50 μM 时的成纤维细胞凋亡（p<0.01）。这表明弹性蛋白肽具有预防和调节皮肤光老化的巨大潜力。
