Zhang Jianan, Liu Yang, Jiang Liwen, Zhao Tiantian, Su Guowan, Zhao Mouming
School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.
Guangdong Food Green Processing and Nutrition Regulation Technologies Research Center, Guangzhou 510650, China.
Molecules. 2023 Nov 10;28(22):7534. doi: 10.3390/molecules28227534.
To enhance the understanding of enzymatic hydrolysis and to accelerate the discovery of key bioactive peptides within enzymatic products, this research focused on elastin as the substrate and investigated the variations in peptide profiles and the production of key bioactive peptides (those exceeding 5% of the total) and their impacts on the biological activity of the hydrolysates. Through the application of advanced analytical techniques, such as stop-flow two-dimensional liquid chromatography and ultra-high-performance liquid chromatography-tandem mass spectrometry, the research tracks the release and profiles of peptides within elastin hydrolysates (EHs). Despite uniform peptide compositions, significant disparities in peptide concentrations were detected across the hydrolysates, hinting at varying levels of bioactive efficacy. A comprehensive identification process pinpointed 403 peptides within the EHs, with 18 peptides surpassing 5% in theoretical maximum content, signaling their crucial role in the hydrolysate's bioactivity. Of particular interest, certain peptides containing sequences of alanine, valine, and glycine were released in higher quantities, suggesting Alcalase 2.4L's preference for these residues. The analysis not only confirms the peptides' dose-responsive elastase inhibitory potential but also underscores the nuanced interplay between peptide content, biological function, and their collective synergy. The study sets the stage for future research aimed at refining enzymatic treatments to fully exploit the bioactive properties of elastin.
为了增进对酶促水解的理解，并加速在酶解产物中发现关键生物活性肽，本研究聚焦于弹性蛋白作为底物，研究了肽谱的变化、关键生物活性肽（占总量超过5%的肽）的产生及其对水解产物生物活性的影响。通过应用先进的分析技术，如停流二维液相色谱和超高效液相色谱-串联质谱，该研究追踪了弹性蛋白水解产物（EHs）中肽的释放和谱图。尽管肽的组成均匀，但在不同的水解产物中检测到肽浓度存在显著差异，这暗示了生物活性功效水平的不同。一个全面的鉴定过程确定了EHs中的403种肽，其中18种肽的理论最大含量超过5%，表明它们在水解产物生物活性中起关键作用。特别值得注意的是，某些含有丙氨酸、缬氨酸和甘氨酸序列的肽释放量更高，这表明碱性蛋白酶2.4L对这些残基有偏好。分析不仅证实了肽的剂量依赖性弹性蛋白酶抑制潜力，还强调了肽含量、生物学功能及其协同作用之间的细微相互作用。该研究为未来旨在优化酶处理以充分利用弹性蛋白生物活性特性的研究奠定了基础。
