转座元件的分子生物学

Molecular biology of transposable elements.

作者信息

Schmitt R

出版信息

J Antimicrob Chemother. 1986 Oct;18 Suppl C:25-34. doi: 10.1093/jac/18.supplement_c.25.

DOI:10.1093/jac/18.supplement_c.25

Abstract

Transposable elements play an essential part in the epidemic spread of antibiotic resistances among bacterial populations. Their ubiquity and their role in the molecular evolution of genomes and organisms has captured the interest of molecular biologists, who investigate the genetic structure and novel mechanisms of recombination underlying transposition. This article, after introducing the general structure of mobile genetic elements and basic mechanistic concepts of transposition in bacteria, uses the well-studied Tn3 family as paradigm for gene organization, gene function and gene regulation. Finally, new applications of transposons in genetic engineering will be described.

摘要

转座元件在细菌群体中抗生素抗性的流行传播中起着至关重要的作用。它们的普遍性以及在基因组和生物体分子进化中的作用引起了分子生物学家的兴趣，这些分子生物学家研究转座背后的遗传结构和新的重组机制。本文在介绍了移动遗传元件的一般结构和细菌中转座的基本机制概念之后，以研究充分的Tn3家族作为基因组织、基因功能和基因调控的范例。最后，将描述转座子在基因工程中的新应用。