Higashi Yoshihiro, Higashi Kentaro, Mori Asami, Sakamoto Kenji, Ishii Kunio, Nakahara Tsutomu
Department of Molecular Pharmacology, Kitasato University School of Pharmaceutical Sciences.
Biol Pharm Bull. 2018;41(10):1586-1592. doi: 10.1248/bpb.b18-00328.
Resveratrol, which is a polyphenol found in grapes, peanuts, and other plants, has health benefits for various chronic diseases. The aim of the present study was to examine the effect of resveratrol on cataract formation in diabetic rats. Male Wistar rats (7-week-old) were treated with streptozotocin, and the streptozotocin-treated animals were administered 5% D-glucose in drinking water to promote the formation of cataracts by inducing severe hyperglycemia. Resveratrol supplementation (10 or 30 mg/kg/d) in drinking water was initiated immediately after induction of diabetes was confirmed. The full lens images of the horizontal plane were captured with the digital camera system which we developed. Cataract formation was assessed by an observer-based scoring method and by quantitative analysis of digital images of the lens. Cataracts at the peripheral region of the lens were detected 2 weeks after induction of hyperglycemia and progressed depending on the length of the diabetic period. The majority of them developed severe cataracts after 9 weeks of hyperglycemia. Resveratrol did not prevent the appearance of diabetic cataracts but significantly delayed the progression of cataracts compared with controls. The contents of sorbitol and protein carbonyls in lenses of diabetic rats were higher than those of control rats. Resveratrol suppressed the increase in protein carbonyls, but not of sorbitol, in diabetic lenses. These results suggest that resveratrol delays the progression of diabetic cataracts partially through attenuation of oxidative damage to lens proteins. Resveratrol may be beneficial in preventing the progression of diabetic cataracts.
白藜芦醇是一种存在于葡萄、花生和其他植物中的多酚，对多种慢性疾病具有健康益处。本研究的目的是检测白藜芦醇对糖尿病大鼠白内障形成的影响。选用7周龄雄性Wistar大鼠，用链脲佐菌素进行处理，对经链脲佐菌素处理的动物给予饮用水中的5% D -葡萄糖，通过诱导严重高血糖来促进白内障的形成。在确认糖尿病诱导后，立即开始在饮用水中补充白藜芦醇（10或30mg/kg/d）。使用我们开发的数码相机系统拍摄晶状体水平面的完整图像。通过基于观察者的评分方法和对晶状体数字图像的定量分析来评估白内障的形成。在高血糖诱导2周后，在晶状体周边区域检测到白内障，并根据糖尿病病程的长短而进展。在高血糖9周后，大多数白内障发展为严重白内障。与对照组相比，白藜芦醇并不能预防糖尿病性白内障的出现，但能显著延缓白内障的进展。糖尿病大鼠晶状体中山梨醇和蛋白质羰基的含量高于对照大鼠。白藜芦醇抑制了糖尿病晶状体中蛋白质羰基的增加，但没有抑制山梨醇的增加。这些结果表明，白藜芦醇可能部分通过减轻晶状体蛋白的氧化损伤来延缓糖尿病性白内障的进展。白藜芦醇可能有助于预防糖尿病性白内障的进展。
