Kwak HyeongSeob, An Hongyan, Alam Md Badrul, Choi Won-Sik, Lee Sang Yong, Lee Sang-Han
Department of Food Science & Biotechnology, Kyungpook National University.
Food & Bio-Industry Research Institute, Kyungpook National University.
Biol Pharm Bull. 2018;41(10):1606-1610. doi: 10.1248/bpb.b18-00251.
β-Escin, a natural triterpene saponin was extracted from Aesculus hippocastanum seeds, which have been widely used to treat inflammation in traditional medicine. In an effort to study the possible anti-tumor effects of β-escin, we performed wound healing, invasion, and adhesion assays to examine the effects of β-escin on cell migration, invasion, and angiogenesis. Our results revealed that β-escin inhibits cell migration as well as motility in B16F10 and SK-MEL5 cells in a dose-dependent manner. RT-PCR and Western blot analysis showed that β-escin increased TIMP-1, -2 while significantly downregulated phosphorylated extracellular signal-regulated kinase (p-ERK) expression, and suppressing nuclear factor-kappa B (NF-κB) and inhibitor of nuclear factor-kappa B (IκB) expression. Overall, the data from the current study suggest that β-escin has the potential for inhibiting both metastatic and angiogenic activities, and are the earliest evidence for the involvement of the NF-κB/IκB signaling in β-escin-induced anti-tumor effects.
β-七叶皂苷是一种从欧洲七叶树种子中提取的天然三萜皂苷，在传统医学中已被广泛用于治疗炎症。为了研究β-七叶皂苷可能的抗肿瘤作用，我们进行了伤口愈合、侵袭和黏附实验，以检测β-七叶皂苷对细胞迁移、侵袭和血管生成的影响。我们的结果显示，β-七叶皂苷以剂量依赖性方式抑制B16F10和SK-MEL5细胞的迁移及运动能力。逆转录-聚合酶链反应（RT-PCR）和蛋白质免疫印迹分析表明，β-七叶皂苷增加组织金属蛋白酶抑制因子-1（TIMP-1）、-2的表达，同时显著下调磷酸化细胞外信号调节激酶（p-ERK）的表达，并抑制核因子κB（NF-κB）及核因子κB抑制蛋白（IκB）的表达。总体而言，本研究数据表明β-七叶皂苷具有抑制转移和血管生成活性的潜力，并且是NF-κB/IκB信号传导参与β-七叶皂苷诱导的抗肿瘤作用的最早证据。
