Xiao Lifu, Sloan-Dennison Sian, Schultz Zachary D
Department of Chemistry and Biochemistry, Ohio State University, W 18th Ave, Columbus, OH USA 43210.
Proc SPIE Int Soc Opt Eng. 2018 Aug;10726. doi: 10.1117/12.2321300. Epub 2018 Sep 5.
Our lab has shown that nanoparticles functionalized with short peptides can selectively bind to receptor proteins . Our results indicate that the Raman signals observed from purified receptors in surface enhanced Raman scattering (SERS) experiments match those observed with tip-enhanced Raman scattering (TERS) experiments performed on membrane receptors in intact cell membranes. Analysis of the observed Raman signals suggest the signals arise from the amino-acids in the protein receptor responsible for binding and recognition of the ligand attached to the nanoparticle probe. Further experiments show the variance in the data correlates with affinity of the nanoparticle probe with a specific receptor. This result illustrates a new approach to studying membrane receptors.
我们实验室已表明，用短肽功能化的纳米颗粒可以选择性地结合受体蛋白。我们的结果表明，在表面增强拉曼散射（SERS）实验中从纯化受体观察到的拉曼信号与在完整细胞膜中的膜受体上进行的针尖增强拉曼散射（TERS）实验中观察到的信号相匹配。对观察到的拉曼信号的分析表明，这些信号来自蛋白质受体中负责结合和识别附着在纳米颗粒探针上的配体的氨基酸。进一步的实验表明，数据中的差异与纳米颗粒探针与特定受体的亲和力相关。这一结果说明了一种研究膜受体的新方法。
