Clarkson B H, Hall D L, Heilman J R, Wefel J S
J Oral Pathol. 1986 Sep;15(8):423-9. doi: 10.1111/j.1600-0714.1986.tb00651.x.
This study investigates enzyme degradation of collagen at various pH levels and is designed to obtain information pertaining to the effect of proteolytic enzymes on caries-like lesion formation and progression. One hundred and eight sections of human teeth with exposed areas on enamel and root face, were placed into an enzyme/buffer system maintained at pH levels of 4.0, 5.5 and 7.0. Periodically, throughout the experimental period, sections were removed and examined histologically for caries-like lesion formation. Results showed consistent surface erosion occurring on the root face only at a pH level of 5.5 in buffer containing enzymes. Sections placed in pH 5.5 buffer only, which served as controls, showed obvious lesion formation on the root face, but with histologically intact surfaces. This interaction occurring between enzymatic activity and acid demineralization suggests that enzymes may have a contributory effect on caries lesion formation in root surfaces.
本研究调查了在不同pH水平下胶原蛋白的酶降解情况，旨在获取有关蛋白水解酶对龋样病变形成和进展影响的信息。108个牙釉质和牙根面有暴露区域的人类牙齿切片，被置于pH值分别为4.0、5.5和7.0的酶/缓冲液系统中。在整个实验期间定期取出切片，并进行组织学检查以观察龋样病变的形成。结果显示，仅在含有酶的pH值为5.5的缓冲液中，牙根面出现了一致的表面侵蚀。仅置于pH 5.5缓冲液中的切片作为对照，牙根面出现了明显的病变形成，但表面组织学完整。酶活性与酸脱矿之间的这种相互作用表明，酶可能对牙根表面龋损的形成有促进作用。
