National Engineering Research Centre for Nanotechnology, Shanghai 200241, People's Republic of China; School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.
National Engineering Research Centre for Nanotechnology, Shanghai 200241, People's Republic of China.
Mater Sci Eng C Mater Biol Appl. 2018 Dec 1;93:1054-1063. doi: 10.1016/j.msec.2018.08.058. Epub 2018 Aug 30.
Folate receptor targeted photo-luminescent quantum carbon dots (Fr-CDs) were successfully prepared from folic acid and phenylenediamine isomers through hydrothermal approaches. Fr-CDs were spherical particles smaller than 10 nm, and emit stable green, blue and red luminescence under ultraviolet region excitation (λex = 365 nm) with maximum emissive lengths at 530, 429, and 612 nm. And the corresponding photoluminescence quantum yield as 15.4%, 12.6% and 16.2% respectively. Up-converted photoluminescent properties in near infrared 800 nm spectral region located in green, blue and yellow region. In-vitro studies showed Fr-CDs had almost none cytotoxicity (cell viability over 80%) and high affinitive to the Hela celline highly-expressed-folate-receptor membranes, and lighted on cytoplasm as the fluorescent marker. It displayed long luminescent-stability with PL intensity above 90% in ultraviolet illuminant exposure over 24 h. In in-vivo studies, Fr-CDs were internalized and accumulated in targeted cancer tissues of cervical carcinoma and the emitting fluorescence maintains over 30 min.
叶酸受体靶向光致发光量子点（Fr-CDs）通过水热法成功地从叶酸和苯二胺异构体中制备。Fr-CDs 是小于 10nm 的球形颗粒，在紫外区域激发（λex=365nm）下发射稳定的绿色、蓝色和红色荧光，最大发射长度分别为 530nm、429nm 和 612nm。相应的光致发光量子产率分别为 15.4%、12.6%和 16.2%。上转换光致发光性能位于近红外 800nm 光谱区域，位于绿色、蓝色和黄色区域。体外研究表明，Fr-CDs 几乎没有细胞毒性（细胞活力超过 80%），对高表达叶酸受体的 Hela 细胞系细胞膜具有高亲和力，并作为荧光标记点亮细胞质。在 24 小时的紫外照射下，其光稳定性保持在 90%以上。在体内研究中，Fr-CDs 被内化并在宫颈癌的靶向癌组织中积累，发射荧光保持超过 30 分钟。
