乳腺癌患者中癌/睾丸抗原KK-LC-1蛋白的检测
Detection of KK-LC-1 Protein, a Cancer/Testis Antigen, in Patients with Breast Cancer.
作者信息
Kondo Yasushi, Fukuyama Takashi, Yamamura Rui, Futawatari Nobue, Ichiki Yoshinobu, Tanaka Yoichi, Nishi Yatsushi, Takahashi Yoshihito, Yamazaki Hitoshi, Kobayashi Noritada, Watanabe Masahiko
机构信息
Department of Surgery, School of Medicine, Kitasato University, Sagamihara, Japan.
Division of Surgery, Kitasato University Medical Center, Kitamoto, Japan.
出版信息
Anticancer Res. 2018 Oct;38(10):5923-5928. doi: 10.21873/anticanres.12937.
BACKGROUND
Kita-Kyushu lung cancer antigen-1 (KK-LC-1) is a cancer/testis antigen and predominant target for cancer immunotherapy. Its detection is only established based on gene expression. In this study, we established a monoclonal antibody against KK-LC-1 to detect its protein expression in formalin-fixed samples.
MATERIALS AND METHODS
The monoclonal antibody against KK-LC-1 was evaluated and the detection of KK-LC-1 between gene expression and protein expression was compared in patients with breast cancer. The monoclonal antibody clone 34B3, which we established, stained testicular germ cells positively.
RESULTS
The rates of detection of KK-LC-1 gene and protein expression were 11.8% and 52.9%, respectively. Protein expression was detected in all triple-negative breast cancer cases studied (n=8). Furthermore, KK-LC-1 was detected in all tumours without oestrogen receptor expression.
CONCLUSION
This study indicated that KK-LC-1 expression was detected in breast cancer, especially in oestrogen receptor-negative subtypes.
背景
北九州肺癌抗原-1（KK-LC-1）是一种癌胚抗原，也是癌症免疫治疗的主要靶点。其检测仅基于基因表达。在本研究中，我们制备了一种抗KK-LC-1的单克隆抗体，以检测其在福尔马林固定样本中的蛋白表达。
材料与方法
对所制备的抗KK-LC-1单克隆抗体进行评估，并比较乳腺癌患者中KK-LC-1基因表达与蛋白表达的检测情况。我们制备的单克隆抗体克隆34B3对睾丸生殖细胞呈阳性染色。
结果
KK-LC-1基因和蛋白表达的检测率分别为11.8%和52.9%。在所研究的所有三阴性乳腺癌病例（n = 8）中均检测到蛋白表达。此外，在所有无雌激素受体表达的肿瘤中均检测到KK-LC-1。
结论
本研究表明，乳腺癌中可检测到KK-LC-1表达，尤其是在雌激素受体阴性亚型中。
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