新型单克隆抗体检测胃癌中 Kita-Kyushu 肺癌抗原-1 的表达。
Expression of Kita-Kyushu Lung Cancer Antigen-1 as Detected by a Novel Monoclonal Antibody in Gastric Cancer.
机构信息
Department of Surgery, Kitasato University Medical Center, Kitamoto, Japan.
Department of General, Pediatric and Hepatobiliary-Pancreatic Surgery, Kitasato University, Sagamihara, Japan.
出版信息
Anticancer Res. 2019 Nov;39(11):6259-6263. doi: 10.21873/anticanres.13835.
BACKGROUND/AIM: Kita-Kyushu lung cancer antigen-1 (KK-LC-1) is a known cancer/testis antigen. Our group has previously shown KK-LC-1 gene expression in gastric cancer. However, could not be detected the KK-LC-1 protein due to the lack of an appropriate antibody. Here, we assessed our original monoclonal antibody (Kmab34B3) and, using it, assessed the expression of KK-LC-1 in gastric cancer.
PATIENTS AND METHODS
We evaluated an original monoclonal antibody against KK-LC-1 (Kmab34B3), and used this antibody to compare KK-LC-1 protein expression in tumour and non-tumour stomach cells from gastric cancer patients.
RESULTS
Kmab34B3 stained testicular germ cells, and tumour cells in nine out of 11 (82%) specimens. In non-tumorous areas, Kmab34B3 stained 13 out of 29 (45%) pyloric gland specimens. Furthermore, Kmab34B3 also stained intestinal metaplasia positive and negative areas.
CONCLUSION
Kmab34B3 was able to detect KK-LC-1 protein within tumour cells and the pyloric gland where the gene has been shown to be expressed. Therefore, it might be an attractive tool for detecting KK-LC-1 expression in precancerous and cancerous stomach cells.
背景/目的：Kita-Kyushu 肺癌抗原-1（KK-LC-1）是一种已知的癌症/睾丸抗原。我们的研究小组之前已经在胃癌中检测到 KK-LC-1 基因的表达。然而，由于缺乏合适的抗体，无法检测到 KK-LC-1 蛋白。在这里，我们评估了我们的原始单克隆抗体（Kmab34B3），并使用它评估了胃癌中 KK-LC-1 的表达。
患者和方法
我们评估了一种针对 KK-LC-1 的原始单克隆抗体（Kmab34B3），并使用该抗体比较了来自胃癌患者的肿瘤和非肿瘤胃细胞中 KK-LC-1 蛋白的表达。
结果
Kmab34B3 染色了 11 个标本中的 9 个（82%）睾丸生殖细胞和肿瘤细胞。在非肿瘤区域，Kmab34B3 染色了 29 个标本中的 13 个（45%）幽门腺。此外，Kmab34B3 还染色了肠上皮化生阳性和阴性区域。
结论
Kmab34B3 能够在肿瘤细胞和已经显示基因表达的幽门腺中检测到 KK-LC-1 蛋白。因此，它可能是一种用于检测癌前和癌变胃细胞中 KK-LC-1 表达的有吸引力的工具。
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