Complete genome sequence of BT304, isolated from the small intestine of castrated beef cattle.

BACKGROUND

Members of the species , belonging to the family in the phylum , have been isolated from a wide variety of natural sources, such as soil, freshwater, fish gut, and clinical specimens. . is important from an industrial viewpoint, because the bacterium grows rapidly with high cell density and exhibits robustness at various growth conditions. However, the bacterium is an opportunistic pathogen involved in human infections. Here, we sequenced and analyzed the genome of the . strain BT304, isolated from the small intestine of adult castrated beef cattle.

RESULTS

The genome of . BT304 consisted of a single circular chromosome of 2,763,150 bp with a GC content of 71.2%. The genome contained 2359 coding sequences, 51 tRNA genes, and 9 rRNA genes. Sequence annotations with the RAST server revealed many genes related to amino acid, carbohydrate, and protein metabolism. Moreover, the genome contained genes related to branched chain amino acid biosynthesis and degradation. Analysis of the OrthoANI values revealed that the genome has high similarity (> 97.8%) with other . strains, such as DC2201, FDAARGOS 302, and G2. Comparative genomic analysis further revealed that the antibiotic properties of . vary among the strains.

CONCLUSION

The relatively small number of virulence-related genes and the great potential in production of host available nutrients suggest potential application of the BT304 strain as a probiotic in breeding beef cattle.