Wagner H, Jordan E, Feil B
Oncology. 1986;43 Suppl 1:16-22. doi: 10.1159/000226416.
The newly isolated phenylpropanoids syringin, syringenin-apiosylglucoside, eleutheroside E and the high molecular lectins and viscotoxins were selected for a standardization of mistletoe extracts and drug preparation. The phenylpropanoids found in all alcoholic and aqueous extracts were suitable for an HPLC fingerprint analysis, and for quantitative determination. The lectin content of the drug preparations was determined by single radial immunodiffusion. As shown by the isoelectric focussing method, Iscador and fermented mistletoe extracts contain only the mistletoe lectins ML II/III, whereas the proteins of the ML I complex are missing. For identification and quantitative determination of viscotoxins, an HPLC method was designed.
新分离出的苯丙素类化合物紫丁香苷、紫丁香苷 - 芹菜糖基葡萄糖苷、刺五加苷E以及高分子凝集素和槲寄生毒素被选用于槲寄生提取物和药物制剂的标准化。在所有醇提取物和水提取物中发现的苯丙素类化合物适用于高效液相色谱指纹图谱分析和定量测定。药物制剂中的凝集素含量通过单向放射免疫扩散法测定。等电聚焦法显示，Iscador和发酵槲寄生提取物仅含有槲寄生凝集素ML II/III，而ML I复合物的蛋白质缺失。为了鉴定和定量测定槲寄生毒素，设计了一种高效液相色谱法。
