Jordan E, Wagner H
Arzneimittelforschung. 1986 Mar;36(3):428-33.
Mistletoe lectins and viscotoxins, which up to now have been isolated only from plant material, were detected and quantitatively determined in the mistletoe preparation Iscador and in a fermented mistletoe extract. Lectins were isolated by affinity chromatography and analyzed by isoelectric focussing. Thus, in Iscador and in the fermented mistletoe extract only the mistletoe lectins ML II/III were found whereas typical proteins of the ML I complex were missing. The lectin content of the preparation and the extract was determined by "single radial immunodiffusion" (SRID). For identification and quantitative determination of viscotoxins, a HPLC method was designed.
至今仅从植物材料中分离得到的槲寄生凝集素和槲寄生毒素，在槲寄生制剂Iscador和一种发酵槲寄生提取物中被检测到并进行了定量测定。凝集素通过亲和层析法分离，并通过等电聚焦进行分析。因此，在Iscador和发酵槲寄生提取物中仅发现了槲寄生凝集素ML II/III，而ML I复合物的典型蛋白质缺失。制剂和提取物中的凝集素含量通过“单向辐射免疫扩散”（SRID）测定。为了鉴定和定量测定槲寄生毒素，设计了一种高效液相色谱法。
