de Alencar Fernandes Neto José, Nonaka Cassiano Francisco Weege, de Vasconcelos Catão Maria Helena Chaves
Postgraduate Program in Dentistry, State University of Paraíba, R. Baraúnas, 351, Bodocongó, Campina Grande, PB, 58429-500, Brazil.
Lasers Med Sci. 2019 Jun;34(4):721-728. doi: 10.1007/s10103-018-2647-x. Epub 2018 Oct 1.
The aim of this study was to evaluate the effects of blue light-emitting diode (LED) on the healing process of third-degree skin burns in rats through clinical and histological parameters. Forty male Wistar rats were divided into two groups: control (CTR) (n = 20) and blue LED (BLUE) (n = 20), with subgroups (n = 5) for each time of euthanasia (7, 14, 21, and 28 days). LED (470 nm, 1 W, 12.5 J/cm per point, 28 s) was applied at four points of the wound (total, 50 J/cm). Feed intake was measured every other day. It was observed that there were no statistically significant differences in the Wound Retention Index (WRI) of the BLUE group in relation to CTR group (p > 0.05) at the evaluation times. After 14, 21, and 28 days, it was observed that the animals in the BLUE group consumed more feed than animals in the CTR group (p < 0.05). At 7 days, there was a statistically significant increase in the angiogenic index (AI) in BLUE (median: 6.2) when compared to CTR (median: 2.4) (p = 0.01) and all animals in BLUE had already started re-epithelialization. This study suggests that blue LED, at the dosimetry used, positively contributed in important and initial stages of the healing process of third-degree skin burns.
本研究旨在通过临床和组织学参数评估蓝光发光二极管（LED）对大鼠三度皮肤烧伤愈合过程的影响。40只雄性Wistar大鼠分为两组：对照组（CTR）（n = 20）和蓝光LED组（BLUE）（n = 20），每组又根据安乐死时间（7、14、21和28天）分为亚组（n = 5）。在伤口的四个点施加LED（470nm，1W，每点12.5J/cm²，28秒）（总计50J/cm²）。每隔一天测量一次进食量。在评估时间点，观察到BLUE组的伤口保留指数（WRI）与CTR组相比无统计学显著差异（p > 0.05）。在14、21和28天后，观察到BLUE组的动物比CTR组的动物进食量更多（p < 0.05）。在7天时，与CTR组（中位数：2.4）相比，BLUE组的血管生成指数（AI）有统计学显著增加（中位数：6.2）（p = 0.01），并且BLUE组的所有动物都已开始重新上皮化。本研究表明，在所使用的剂量下，蓝光LED在三度皮肤烧伤愈合过程的重要初始阶段起到了积极作用。
