骨PG II cDNA的特性及其与其他结缔组织中PG II mRNA的关系。

Characterization of bone PG II cDNA and its relationship to PG II mRNA from other connective tissues.

作者信息

Day A A, Ramis C I, Fisher L W, Gehron-Robey P, Termine J D, Young M F

出版信息

Nucleic Acids Res. 1986 Dec 22;14(24):9861-76. doi: 10.1093/nar/14.24.9861.

DOI:10.1093/nar/14.24.9861

PMID:3027667

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC341340/

Abstract

Two cDNA clones encoding the small proteoglycan II (PG II) of bone were isolated from a lambda gt11 expression library. These clones expressed recombinant protein which was cross-reactive with polyclonal and monoclonal antisera to PG II molecules from several connective tissues. The longest clone, lambda Pg 20 was studied in detail. The clone was shown to encode PG II by hybrid selected translation and immunoprecipitation. Northern analysis showed two species of the PG II message of approximately 1.4 and 1.8 kb. Substantial amounts of PG II message were found in bone, tendon, articular cartilage, skin, smooth muscle and cornea. Trace amounts of message were also detected in liver and brain. Radiolabeled bovine PG II cDNA hybridized to RNA from several other species including the human, rat and chicken. The level of PG II mRNA in chick embryonic fibroblasts was sensitive to transformation by Rous sarcoma virus.

摘要

从λgt11表达文库中分离出两个编码骨小蛋白聚糖II（PG II）的cDNA克隆。这些克隆表达的重组蛋白与来自几种结缔组织的PG II分子的多克隆和单克隆抗血清发生交叉反应。对最长的克隆λPg 20进行了详细研究。通过杂交选择翻译和免疫沉淀证明该克隆编码PG II。Northern分析显示有两种PG II信使核糖核酸，大小约为1.4和1.8 kb。在骨、肌腱、关节软骨、皮肤、平滑肌和角膜中发现了大量的PG II信使核糖核酸。在肝脏和大脑中也检测到微量的信使核糖核酸。放射性标记的牛PG II cDNA与来自其他几种物种（包括人、大鼠和鸡）的RNA杂交。鸡胚胎成纤维细胞中PG II mRNA的水平对劳氏肉瘤病毒的转化敏感。