Chen Yun, Liu Liang
School of Pharmacy, Nanjing Medical University, Nanjing, China.
Methods Mol Biol. 2019;1871:265-277. doi: 10.1007/978-1-4939-8814-3_17.
Targeted proteomics detects proteins of interest with high sensitivity, quantitative accuracy, and reproducibility. In a targeted proteomics assay, surrogate peptides are generated by proteolytic digestion of target proteins and selected reaction monitoring (SRM) assays are developed to quantify these peptides using liquid chromatography-tandem mass spectrometry (LC-MS/MS). In this report, we describe the details of quantitative analysis of target protein in cells and tissue samples.
靶向蛋白质组学能够以高灵敏度、定量准确性和可重复性检测感兴趣的蛋白质。在靶向蛋白质组学分析中，通过对目标蛋白质进行蛋白酶解产生替代肽段，并开发选择反应监测（SRM）分析方法，利用液相色谱-串联质谱（LC-MS/MS）对这些肽段进行定量。在本报告中，我们描述了细胞和组织样本中目标蛋白质定量分析的详细情况。
