Cooksey D A
Plasmid. 1986 Nov;16(3):222-4. doi: 10.1016/0147-619x(86)90061-2.
A spontaneous agrocin-resistant mutant of Agrobacterium tumefaciens strain C58 was shown to have an insertion of 1.2 kb in the agrocin-sensitivity region of pTiC58. The insertion was cloned from the Ti-plasmid, and a subclone containing only DNA internal to the insertion was used to probe the Ti-plasmid and chromosomal DNA of the wild-type strain C58. The probe showed homology to chromosomal sequences but showed no homology to wild-type pTiC58. Homology was also detected with chromosomal sequences of A. tumefaciens strains, B6, K24, and T37. These results suggest that an indigenous insertion sequence of 1.2 kb transposed from the chromosome to the agrocin-sensitivity region of the Ti-plasmid in this spontaneous mutant of C58.
根癌土壤杆菌C58菌株的一个自发抗农杆菌素突变体被证明在pTiC58的农杆菌素敏感区域有一个1.2 kb的插入片段。该插入片段从Ti质粒中克隆出来，一个仅包含插入片段内部DNA的亚克隆被用于探测野生型菌株C58的Ti质粒和染色体DNA。该探针与染色体序列显示出同源性，但与野生型pTiC58没有同源性。在根癌土壤杆菌菌株B6、K24和T37的染色体序列中也检测到了同源性。这些结果表明，在C58的这个自发突变体中，一个1.2 kb的内源插入序列从染色体转移到了Ti质粒的农杆菌素敏感区域。
