Adair B M
Res Vet Sci. 1986 Nov;41(3):414-6.
The fluorescent antibody (FA) test is compared with the haemagglutination inhibition (HI) test for parainfluenza virus type 3 (PI-3) and virus neutralisation (VN) test for respiratory syncytial (RS) virus for detection and titration of virus-specific antibodies. In experimentally inoculated calves PI-3 and RS virus FA tests detected seroconversion at the same time as HI and VN tests, however, in serially diluted sera, the FA test was positive to higher dilution. In studies with paired samples from calves from four farms with respiratory problems, the FA test gave similar results to PI-3 HI and RS virus VN tests. Large increases in antibody titre to RS virus detected by FA and VN tests indicated this was the problem on two of the farms. Individual animals showed large rises to PI-3 by FA and HI test on three farms. It is concluded that the FA test provides a rapid and sensitive alternative to the more conventional serological tests for respiratory viruses.
为检测和滴定病毒特异性抗体，对3型副流感病毒（PI-3）的荧光抗体（FA）试验与血凝抑制（HI）试验以及呼吸道合胞病毒（RS）的病毒中和（VN）试验进行了比较。在实验接种的犊牛中，PI-3和RS病毒FA试验与HI和VN试验同时检测到血清转化，然而，在系列稀释血清中，FA试验在更高稀释度时仍呈阳性。在对来自四个有呼吸道问题农场的犊牛配对样本进行的研究中，FA试验对PI-3的HI试验和RS病毒的VN试验给出了相似结果。通过FA和VN试验检测到的RS病毒抗体滴度大幅升高表明这是其中两个农场存在的问题。在三个农场中，通过FA和HI试验检测到个别动物对PI-3的抗体大幅升高。得出的结论是，对于呼吸道病毒，FA试验为更传统的血清学试验提供了一种快速且灵敏的替代方法。
