Department of Rheumatology, Chongqing Hospital of Traditional Chinese Medicine, Chongqing 400021, PR China.
Guangdong Pharmaceutical University, Guangzhou 510006, PR China.
J Ethnopharmacol. 2019 Jan 30;229:73-80. doi: 10.1016/j.jep.2018.09.039. Epub 2018 Sep 29.
Total flavones of Epimedium (TFE) is the main active ingredient in Herba Epimedii, which is a well-known Chinese herbal medicine that is widely used to treat certain age-related diseases in oriental countries.
The aim of this work was to investigate the anti-aging properties of TFE related to lipid metabolism.
Both transcriptomics and metabonomics were applied in this work to investigate the anti-aging properties of TFE. Microarray and LC-MS analysis were conducted on liver samples of three groups of rats, including young (4 months), old (24 months), and old rats administrated TFE.
Transcriptomics analysis highlighted 287 transcripts related to the anti-aging effect of TFE, in which the expression ratio of 18 genes regulating lipid metabolism, including HMGCS1 and NR1H3, returned to normal levels after TFE treatment. In addition, 24 aging-related metabolites were discovered in a metabonomics study, and 15 of these were structurally identified, including palmitic amide, linoleamide, and oleamide. Bioinformatics and integral data analysis on the results of the transcriptomics and metabonomics suggest the involvement of 12 key metabolic pathways, half of which are highly related to lipid metabolism.
This study demonstrates that the role played by TFE in the lipid metabolism of aging rats is multifaceted and multi-layered.
淫羊藿总黄酮（TFE）是淫羊藿中主要的活性成分，淫羊藿是一种在东方国家广泛用于治疗某些与年龄相关疾病的著名中草药。
本研究旨在探讨 TFE 对脂代谢相关的抗衰老特性。
本工作采用转录组学和代谢组学来研究 TFE 的抗衰老特性。对三组大鼠的肝组织进行了微阵列和 LC-MS 分析，包括年轻（4 个月）、年老（24 个月）和给予 TFE 的老年大鼠。
转录组学分析突出了 287 个与 TFE 抗衰老作用相关的转录本，其中调节脂代谢的 18 个基因的表达比例在 TFE 处理后恢复正常水平。此外，在代谢组学研究中发现了 24 个与衰老相关的代谢物，其中 15 个被结构鉴定，包括棕榈酰胺、亚油酸酰胺和油酸酰胺。转录组学和代谢组学结果的生物信息学和整体数据分析表明，涉及 12 个关键代谢途径，其中一半与脂代谢高度相关。
本研究表明，TFE 在衰老大鼠脂代谢中的作用是多方面和多层次的。
