Wu Bin, Yan Shikai, Lin Zhongying, Wang Qi, Yang Yun, Yang Genjin, Shen Ziyin, Zhang Weidong
Institute of Integrative Chinese and Western Medicine, Huashan Hospital, Fudan University, Shanghai 200040, PR China.
Mol Biosyst. 2008 Aug;4(8):855-61. doi: 10.1039/b800923f. Epub 2008 Jun 4.
The aim of this work was to investigate the effects of ageing on rat urinary metabolites and to evaluate the anti-ageing effects of the total flavone of Epimedium (TFE). Proton nuclear magnetic resonance based metabonomic analyses was performed on urine from rats aged 4, 10, 18 and 24 months, and rats administered with TFE. By multivariate analysis, 26 characteristic resonances were found to be highly related with the age of rats, and ten of them were structurally postulated as creatinine, lactate, alanine, acetate, acetone, succinate, allantoin, methylamine, dimethylamine and trimethylamine-N-oxide; these metabolites involve creatinine metabolites, aliphatic amines metabolites and some important intermediates or end products of energy metabolism. Principal components analysis revealed that the metabolic profiles of 24-month-old rats treated with TFE closely resembled those of rats aged 18 months. In addition, most of these characteristic resonances were reset to younger levels by TFE intervention. The result suggests that TFE administration can markedly influence the ageing process and shows anti-ageing effects, which might due to the melioration of pyruyate metabolism and oxidative phosphorylation.
这项工作的目的是研究衰老对大鼠尿液代谢产物的影响，并评估淫羊藿总黄酮（TFE）的抗衰老作用。对4、10、18和24月龄大鼠以及给予TFE的大鼠的尿液进行了基于质子核磁共振的代谢组学分析。通过多变量分析，发现26个特征共振与大鼠年龄高度相关，其中10个在结构上被推测为肌酐、乳酸、丙氨酸、乙酸盐、丙酮、琥珀酸盐、尿囊素、甲胺、二甲胺和氧化三甲胺；这些代谢产物涉及肌酐代谢产物、脂肪族胺代谢产物以及能量代谢的一些重要中间体或终产物。主成分分析显示，用TFE处理的24月龄大鼠的代谢谱与18月龄大鼠的代谢谱非常相似。此外，TFE干预使这些特征共振中的大多数恢复到较年轻的水平。结果表明，给予TFE可显著影响衰老过程并显示出抗衰老作用，这可能是由于丙酮酸代谢和氧化磷酸化的改善。
