The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, 237 Luoyu Road, Wuhan 430079, People's Republic of China.
The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, 237 Luoyu Road, Wuhan 430079, People's Republic of China.
Int J Pharm. 2018 Dec 1;552(1-2):206-216. doi: 10.1016/j.ijpharm.2018.09.066. Epub 2018 Sep 29.
Eliminating the infection from root canals and periodontal tissues of human teeth as well as the repair of infected periodontal bone has been a great challenge. Poly(lactide-co-glycolide) (PLGA) particles have been used for drug delivery due to their biodegradability, sustained drug release and nontoxic properties. As silver ions (Ag) exhibit strong antibacterial ability and calcium ions (Ca) are essential for hard tissue regeneration, the Ag and Ca were encapsulated into the PLGA particles to form AgCa-PLGA submicron particles. The physico-chemical properties, ion release, cytotoxicity, ability to infiltrate dentinal tubules, antibacterial effects against Enterococcus faecalis (E. faecalis) and Porphyromonas gingivalis (P. gingivalis) in either planktonic or biofilm forms on dentin as well as in vitro mineralization ability were investigated. Results showed that Ag and Ca were encapsulated into the AgCa-PLGA particles, which could release both Ag and Ca over 30 days and exhibited strong antibacterial effects against E. faecalis and P. gingivalis. AgCa-PLGA particles could infiltrate into dentinal tubules by ultrasonic activation and inhibit the colonization of E. faecalis and P. gingivalis on dentin. AgCa-PLGA particles showed no cytotoxicity and induced hydroxyapatite (HA)-like crystal formation. AgCa-PLGA submicron particles could become a biomaterial for both infection control and hard tissue regeneration in dentistry and other related medical areas.
消除人牙齿根管和牙周组织中的感染以及修复感染的牙周骨一直是一个巨大的挑战。聚（丙交酯-乙交酯）（PLGA）颗粒由于其可生物降解性、持续药物释放和无毒特性而被用于药物输送。由于银离子（Ag）具有很强的抗菌能力，钙离子（Ca）是硬组织再生所必需的，因此将 Ag 和 Ca 包封到 PLGA 颗粒中，形成 AgCa-PLGA 亚微米颗粒。研究了其物理化学性质、离子释放、细胞毒性、在牙本质中渗透牙本质小管的能力、对粪肠球菌（E. faecalis）和牙龈卟啉单胞菌（P. gingivalis）的浮游或生物膜形式的抗菌作用以及体外矿化能力。结果表明，Ag 和 Ca 被包封到 AgCa-PLGA 颗粒中，这些颗粒可以在 30 天内释放出 Ag 和 Ca，并对 E. faecalis 和 P. gingivalis 表现出很强的抗菌作用。AgCa-PLGA 颗粒可以通过超声激活渗透到牙本质小管中，并抑制 E. faecalis 和 P. gingivalis 在牙本质上的定植。AgCa-PLGA 颗粒无细胞毒性，并诱导羟基磷灰石（HA）样晶体形成。AgCa-PLGA 亚微米颗粒可成为控制感染和硬组织再生的生物材料，用于牙科和其他相关医学领域。
