Duan Mengting, Fan Wei, Fan Bing
The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan 430000, China.
Pharmaceutics. 2021 Sep 19;13(9):1518. doi: 10.3390/pharmaceutics13091518.
Mesoporous calcium-silicate nanoparticles (MCSNs) are excellent biomaterials for controlled drug delivery and mineralization induction. In this study, MCSNs were loaded with low-dose silver ion (Ag) and Triton X-100 (TX-100) as the M-AgTX to achieve both enhanced antibacterial properties and low cytotoxicity for dentin disinfection. The physicochemical property, biocompatibility, infiltration ability into dentinal tubules, anti-bacterial ability against both planktonic () and its biofilm on dentin, effects on dentin microhardness and in vitro mineralization property were systematically investigated. Results confirmed that the MCSNs and M-AgTX nanoparticles showed typical morphology of mesoporous materials and exhibited sustained release of chemicals with an alkaline pH value over time. M-AgTX also exhibited excellent biocompatibility on MC3T3-E1 cells and could eliminate 100% planktonic after 48-h treatment. On dentin slices, it could enter dentinal tubules by ultrasonic activation and inhibit the growth of on dentin. M-AgTX could completely inactive 28-day biofilm. TEM confirmed the destruction of cell membrane integrity and Ag infiltration into bacteria by M-AgTX. Besides, dentin slices medicated with M-AgTX nanoparticles displayed an increased microhardness. After being immersed in SBF for 7 days, apatite crystals could be observed on the surface of the material tablets. M-AgTX could be developed into a new multifunctional intra-canal medication or bone defect filling material for infected bone defects due to its sustained release profile, low cytotoxicity, infiltration ability, enhanced anti-bacterial and mineralization features.
介孔硅酸钙纳米颗粒（MCSNs）是用于控释药物递送和矿化诱导的优异生物材料。在本研究中，低剂量银离子（Ag）和 Triton X - 100（TX - 100）负载于 MCSNs 形成 M - AgTX，以实现增强的抗菌性能和低细胞毒性用于牙本质消毒。系统研究了其物理化学性质、生物相容性、向牙本质小管的渗透能力、对浮游菌（）及其在牙本质上生物膜的抗菌能力、对牙本质显微硬度的影响以及体外矿化性能。结果证实，MCSNs 和 M - AgTX 纳米颗粒呈现介孔材料的典型形态，并随着时间推移呈现化学物质的持续释放且 pH 值呈碱性。M - AgTX 对 MC3T3 - E1 细胞也表现出优异的生物相容性，并且在 48 小时处理后可消除 100%的浮游菌。在牙本质切片上，它可通过超声激活进入牙本质小管并抑制牙本质上细菌的生长。M - AgTX 可使 28 天的生物膜完全失活。透射电子显微镜（TEM）证实 M - AgTX 破坏了细胞膜完整性并使银渗入细菌。此外，用 M - AgTX 纳米颗粒处理的牙本质切片显示显微硬度增加。在模拟体液（SBF）中浸泡 7 天后，在材料片剂表面可观察到磷灰石晶体。由于其持续释放特性、低细胞毒性、渗透能力、增强的抗菌和矿化特性，M - AgTX 可开发成为一种新型多功能根管内用药或用于感染性骨缺损的骨缺损填充材料。
