Engster Maryann S
Department of Biological Sciences, State University of New York, Albany, New York 12222.
J Morphol. 1976 Sep;150(1):183-211. doi: 10.1002/jmor.1051500109.
As part of a study on trichopteran silk secretion, the histology, histochemistry, and ultrastructure of the silk glands of two species of limnephilid trichopteran larvae, Pycnopsyche guttifer (Walk.) and Neophylax concinnus McL., were investigated. The silk glands consist of three anatomically distinct regions: a long, posterior silk-secreting region; a shorter, anterior conducting tube; and a terminal press/common duct. In Pycnopsyche, there is also a modified bulbous region between the secreting and conducting areas. Each anatomical region has a distinct cell type. There are two structurally and histochemically different components of the secretion in the glandular lumen: a core and a peripheral layer. Both components are produced all along the gland and are principally proteinaceous. However, the peripheral layer is also PAS and alcian blue (pH. 2.5) positive and shows β-metachromasia with toluidine blue (pH 3.5), indicating the presence of both neutral and acidic polysaccharides.
作为一项关于毛翅目昆虫丝分泌的研究的一部分，对两种沼石蛾科毛翅目幼虫——饰腹新 Psychopsyche guttifer (Walk.) 和优美新蛉 Neophylax concinnus McL. 的丝腺进行了组织学、组织化学和超微结构研究。丝腺由三个解剖学上不同的区域组成：一个长的、后部的丝分泌区域；一个较短的、前部的传导管；以及一个末端压榨/共同导管。在饰腹新蛉中，在分泌区和传导区之间还有一个改良的球根状区域。每个解剖区域都有独特的细胞类型。腺腔内的分泌物有两种结构和组织化学上不同的成分：一个核心和一个外周层。这两种成分在整个腺体中都有产生，且主要是蛋白质性质的。然而，外周层对PAS和阿尔辛蓝（pH 2.5）呈阳性反应，并用甲苯胺蓝（pH 3.5）显示β异染性，表明存在中性和酸性多糖。
