Sanja Marevic, Jozsef Petrik, Sanja Popovic Grle, Ivana Cepelak, Ivana Grgic, Lana Gorenec, Gordana Stjepanovic, Renata Laskaj, Lepej Snjezana Zidovec
a Department of Medical Biochemistry and Hematology , University Hospital for Infectious Diseases ''Dr. Fran Mihaljevic'' , Zagreb , Croatia.
b Faculty of Pharmacy and Biochemistry, Department of Medical Biochemistry and Hematology , University of Zagreb , Zagreb , Croatia.
Scand J Clin Lab Invest. 2018 Nov-Dec;78(7-8):533-538. doi: 10.1080/00365513.2018.1514464. Epub 2018 Oct 2.
Cytokines are biological response modifiers involved in the pathophysiology of chronic obstructive pulmonary disease (COPD). This study investigated the potential use of cytokines as disease severity biomarkers in COPD patients and the possible effect of statin therapy on cytokine expression. Possible associations between cytokines, body mass index (BMI) and smoking have also been studied. Cytokines IFN-γ, IL-2, IL-12 p70, TNF-α, TNF-β, IL-4, IL-5, IL-6, IL-10, IL-1β and IL-8 were measured in the plasma of 100 clinically stable COPD patients using a fluorescent bead immunoassay on a flow cytometer. When patients were grouped according to Global Initiative for Chronic Obstructive Lung Disease (GOLD) stage (A-D), no significant differences in cytokine concentrations were found (p > .05). Significantly decreased concentrations of IL-1β, IL-2, IL-4, IL-8, IL-10, IL-12p70 and TNF-α were found in COPD patients receiving statin therapy in comparison with COPD patients not receiving statin therapy (p < .05). COPD patients with increased BMI (>25) had decreased IL-2 (p=.038), IL-8 (p = .039) and IL-10 (p = .005) concentrations compared to normal BMI (20-25) patients. Current COPD smokers had increased concentrations of IL-5 (p = .037) compared to former COPD smokers. Hierarchical cluster analysis showed several patterns of measured cytokines in serum of patients with stable COPD. Statin therapy is associated with decreased expression of selected Th1 and Th2 cytokines in COPD, and this effect could be of relevance in COPD patients with increased cardiovascular risk. Concentrations of Th1 and Th2 cytokines in plasma cannot be used as biomarkers of disease severity or progression of COPD.
细胞因子是参与慢性阻塞性肺疾病（COPD）病理生理学的生物反应调节剂。本研究调查了细胞因子作为COPD患者疾病严重程度生物标志物的潜在用途以及他汀类药物治疗对细胞因子表达的可能影响。还研究了细胞因子、体重指数（BMI）和吸烟之间的可能关联。使用流式细胞仪上的荧光微球免疫测定法，对100例临床稳定的COPD患者血浆中的细胞因子IFN-γ、IL-2、IL-12 p70、TNF-α、TNF-β、IL-4、IL-5、IL-6、IL-10、IL-1β和IL-8进行了测量。当根据慢性阻塞性肺疾病全球倡议（GOLD）分期（A-D）对患者进行分组时，未发现细胞因子浓度有显著差异（p＞0.05）。与未接受他汀类药物治疗的COPD患者相比，接受他汀类药物治疗的COPD患者中IL-1β、IL-2、IL-4、IL-8、IL-10、IL-12p70和TNF-α的浓度显著降低（p＜0.05）。与正常BMI（20-25）的患者相比，BMI增加（＞25）的COPD患者IL-2（p = 0.038）、IL-8（p = 0.039）和IL-10（p = 0.005）浓度降低。与既往COPD吸烟者相比，目前仍吸烟的COPD患者IL-5浓度升高（p = 0.037）。层次聚类分析显示了稳定期COPD患者血清中几种测量细胞因子的模式。他汀类药物治疗与COPD中选定的Th1和Th2细胞因子表达降低有关，这种效应可能与心血管风险增加的COPD患者有关。血浆中Th1和Th2细胞因子的浓度不能用作COPD疾病严重程度或进展的生物标志物。
