Gholampour Firouzeh, Sadidi Zahra
Department of Biology, College of Sciences, Shiraz University, Shiraz, Iran.
Department of Biology, College of Sciences, Shiraz University, Shiraz, Iran.
J Surg Res. 2018 Nov;231:224-233. doi: 10.1016/j.jss.2018.05.036. Epub 2018 Jun 22.
Pathogenesis of renal ischemia/reperfusion injury (IRI) involves oxidative stress response in the kidney and remote organs. Both quercetin and remote ischemic perconditioning (RIPerC) can protect partially against IRI. This study determined whether combined quercetin and RIPerC could provide an augmented hepatorenal protection against renal IRI.
I/R was induced by clamping renal arteries for 45 min followed by 24-h reperfusion. RIPerC consisted of four cycles of 2 min of left femoral artery ischemia followed by 3 min of reperfusion administered at the beginning of renal ischemia. Rats were divided into five groups: sham, I/R, RIPerC, quercetin (Q + I/R), and combined quercetin and RIPerC (Q + RIPerC). At the end of reperfusion period, blood, urine, and tissue samples were collected.
I/R caused kidney dysfunction, as proved by significant decrease in creatinine clearance, and a significant increase in liver functional indicators as evidenced by increased plasma alanine aminotransferase and aspartate aminotransferase activity. This was accompanied by a decrease of glutathione peroxidase and catalase activities with an increase of malondialdehyde levels and histological damages in renal and hepatic tissues. Treatment with RIPerC and quercetin reduced all these changes. However, the measure of improvements was enhanced by combined quercetin and RIPerC treatment.
This study demonstrated protective effects of quercetin and RIPerC strategy on the both kidney and liver after renal I/R. The results suggest that combined quercetin and RIPerC provides an enhanced protection against renal IRI by reduction of lipid peroxidation and augmentation of antioxidant systems.
肾缺血/再灌注损伤（IRI）的发病机制涉及肾脏及远处器官的氧化应激反应。槲皮素和远程缺血预处理（RIPerC）均可部分保护机体免受IRI损伤。本研究旨在确定槲皮素与RIPerC联合应用是否能增强对肝肾的保护作用，以抵御肾IRI。
通过夹闭肾动脉45分钟，随后再灌注24小时诱导IRI。RIPerC包括在肾缺血开始时进行四个周期的左股动脉缺血2分钟，随后再灌注3分钟。大鼠分为五组：假手术组、IRI组、RIPerC组、槲皮素组（Q + I/R）和槲皮素与RIPerC联合组（Q + RIPerC）。在再灌注期末，收集血液、尿液和组织样本。
IRI导致肾功能障碍，表现为肌酐清除率显著降低，肝功能指标显著升高，如血浆丙氨酸氨基转移酶和天冬氨酸氨基转移酶活性增加。同时伴有谷胱甘肽过氧化物酶和过氧化氢酶活性降低，丙二醛水平升高，以及肾和肝组织的组织学损伤。RIPerC和槲皮素治疗可减轻所有这些变化。然而，槲皮素与RIPerC联合治疗的改善程度更高。
本研究证明了槲皮素和RIPerC策略对肾IRI后肾脏和肝脏均具有保护作用。结果表明，槲皮素与RIPerC联合应用可通过减少脂质过氧化和增强抗氧化系统，增强对肾IRI的保护作用。
