Department of Thoracic Surgery, Jiangdu People's Hospital of Yangzhou City, Yangzhou, China.
Eur Rev Med Pharmacol Sci. 2018 Sep;22(18):5946-5953. doi: 10.26355/eurrev_201809_15925.
To investigate whether lncRNA H19 can regulate NF1 expression through competitive binding to microRNA-107, thereby participating in the occurrence and development of non-small cell lung cancer (NSCLC).
Expression levels of H19 and NF1 in NSCLC tissues, paracancerous tissues and NSCLC cell lines were detected by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). The binding condition of microRNA-107, H19 and NF1 was detected by dual-luciferase reporter gene assay. Corresponding lentiviruses of H19 were constructed. The regulatory effects of H19 on proliferative and migratory abilities of A549 cells were detected by cell counting kit-8 (CCK-8) and transwell assay, respectively. Rescue experiments were conducted to explore the regulatory interaction between H19 and microRNA-107 in A549 cells.
H19 and NF1 were highly expressed in NSCLC tissues and NSCLC cell lines (A549 and HCC823) than those of controls. Overexpressed H19 increased proliferative and migratory abilities of A549 cells. Dual-luciferase reporter gene assay demonstrated that H19 regulates NF1 expression through competitive binding to microRNA-107, thereafter participating in NSCLC development.
H19 is highly expressed in NSCLC, which promotes NSCLC development by regulating NF1 via competitive binding to microRNA-107.
研究长链非编码 RNA H19 是否可以通过与 microRNA-107 竞争结合来调节 NF1 的表达，从而参与非小细胞肺癌（NSCLC）的发生和发展。
通过实时荧光定量聚合酶链反应（qRT-PCR）检测 NSCLC 组织、癌旁组织和 NSCLC 细胞系中 H19 和 NF1 的表达水平。通过双荧光素酶报告基因检测 microRNA-107、H19 和 NF1 的结合情况。构建相应的 H19 慢病毒。通过细胞计数试剂盒-8（CCK-8）和 Transwell 实验分别检测 H19 对 A549 细胞增殖和迁移能力的调节作用。进行挽救实验以探讨 H19 和 microRNA-107 在 A549 细胞中的调节相互作用。
H19 和 NF1 在 NSCLC 组织和 NSCLC 细胞系（A549 和 HCC823）中的表达高于对照组。过表达 H19 增加了 A549 细胞的增殖和迁移能力。双荧光素酶报告基因检测表明，H19 通过与 microRNA-107 竞争结合来调节 NF1 的表达，进而参与 NSCLC 的发生发展。
H19 在 NSCLC 中高表达，通过与 microRNA-107 竞争结合来调节 NF1，从而促进 NSCLC 的发展。
