Muhammad F, Hussain J, Fareed S K, Ahmad Khan T, Ahmad Khan S, Ahmad A
Department of Microbiology, Faculty of Science, University of Karachi, Karachi, Pakistan.
Department of Microbiology, Faculty of Life Sciences & Informatics Balochistan University of IT, Engineering & Management Sciences (BUITEMS), Quetta Takkatu Campus, Airport road Quetta, Balochistan, Pakistan.
Arch Razi Inst. 2018 Sep;73(3):239-244. doi: 10.22092/ari.2017.108217.1085. Epub 2018 Sep 1.
Mycoplasma gallisepticum and Mycoplasma synoviae are the causative agents of avian mycoplasmosis in commercial poultry. Among the available tools, polymerase chain reaction (PCR) and culture are confirmatory tools for the diagnosis of mycoplasmosis after the initial serological screening of suspected birds. Overall, 181 samples were analyzed, 152 (84%) and 103 (57%) of which were found positive by PCR and culture, respectively. Further, 54 (92%) broiler samples were found positive for general avian mycoplasma. Among the total positive samples, MS positivity was as high as 72 (47%) by PCR, while it was 45 (44%) by culture. MG positivity was 23% and 25% in PCR- and culture-positive samples. MG grows more easily compared to MS. The agreement value between the tests was 67%. Overall, flock wise prevalence was not much varied. The prevalence of mycoplasmosis was higher during winter. Our study confirmed that PCR is the most sensitive and reliable tool for the diagnosis of avian mycoplasmosis in field samples.
鸡毒支原体和滑液支原体是商品家禽中禽支原体病的病原体。在现有检测手段中，聚合酶链反应（PCR）和培养是在对疑似禽类进行初步血清学筛查后确诊支原体病的检测方法。总体而言，共分析了181份样本，其中分别有152份（84%）和103份（57%）通过PCR和培养检测呈阳性。此外，54份（92%）肉鸡样本检测出一般禽支原体呈阳性。在所有阳性样本中，通过PCR检测出滑液支原体阳性率高达72份（47%），而通过培养检测出阳性率为45份（44%）。在PCR和培养检测呈阳性的样本中，鸡毒支原体阳性率分别为23%和25%。鸡毒支原体比滑液支原体更容易生长。两种检测方法的一致性值为67%。总体而言，按鸡群计算的患病率差异不大。支原体病在冬季的患病率较高。我们的研究证实，PCR是诊断野外样本中禽支原体病最敏感、最可靠的工具。
