Meinke Stephan, Wikman Agneta, Gryfelt Gunilla, Hultenby Kjell, Uhlin Michael, Höglund Petter, Sandgren Per
Department of Medicine Huddinge, Center for Hematology and Regenerative Medicine (HERM), Karolinska Institutet.
Department of Clinical Immunology and Transfusion Medicine (KITM), Karolinska University Hospital.
Transfusion. 2018 Nov;58(11):2657-2668. doi: 10.1111/trf.14905. Epub 2018 Oct 3.
Cryopreserved platelets (CPPs) are considered a promising approach for extended platelet storage, bridging inventory shortages of conventionally stored platelets. It is unknown if platelet concentrates exposed to photochemical treatment (PCT) with amotosalen and ultraviolet A (UVA) light, to inactivate pathogens, are suitable for freezing. The objective of this study was to analyze potential effects of PCT on CPPs as compared with untreated CPPs.
A total of 12 PCT-treated and 12 untreated platelet units from buffy coats were cryopreserved at -80°C in 5% dimethyl sulfoxide. CPPs of both types were rapidly thawed at 37°C and resuspended in 200 mL fresh plasma. In vitro properties were analyzed prefreezing, postfreezing and thawing, and on Day 1 after thawing.
Directly after thawing, no major differences in platelet content, lactase hydrogenase, adenosine triphosphate, mitochondrial membrane potential, CD62P, CD42b, and platelet endothelial cell adhesion molecule were seen between PCT-CPPs and conventional CPPs. Agonist-induced PAC-1 expression and contribution of CPPs to blood coagulation in an experimental rotational thromboelastometry setup were also similar between the groups. On Day 1 after thawing, the CPPs of both types performed less well. The PCT-CPPs tended to be more affected by the freezing process than the conventional CPPs.
PCT-CPPs appeared slightly more susceptible to lesion effects by freezing than conventional CPPs, in particular in assays on Day 1 after thawing, but these differences were small relative to the dramatic effects of the freezing process itself.
冷冻保存的血小板（CPPs）被认为是延长血小板储存时间的一种有前景的方法，可弥补传统储存血小板的库存短缺。目前尚不清楚经氨甲环酸和紫外线A（UVA）光进行光化学处理（PCT）以灭活病原体的血小板浓缩物是否适合冷冻。本研究的目的是分析与未处理的CPPs相比，PCT对CPPs的潜在影响。
从 Buffy 层中获取总共 12 个经 PCT 处理和 12 个未处理的血小板单位，在-80°C 下于 5%二甲基亚砜中冷冻保存。两种类型的 CPPs 在 37°C 下快速解冻，并重悬于 200 mL 新鲜血浆中。在冷冻前、冷冻后和解冻后以及解冻后第 1 天分析体外特性。
解冻后立即观察到，PCT-CPPs 和传统 CPPs 在血小板含量、乳糖脱氢酶、三磷酸腺苷、线粒体膜电位、CD62P、CD42b 和血小板内皮细胞黏附分子方面没有重大差异。在实验性旋转血栓弹力图设置中，激动剂诱导的 PAC-1 表达以及 CPPs 对血液凝固作用在两组之间也相似。解冻后第 1 天，两种类型的 CPPs 表现均较差。与传统 CPPs 相比，PCT-CPPs 似乎更容易受到冷冻过程的影响。
PCT-CPPs 在冷冻损伤效应方面似乎比传统 CPPs 略更敏感，特别是在解冻后第 1 天的检测中，但相对于冷冻过程本身的显著影响而言，这些差异较小。
