Department of Veterinary Microbiology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
Department of Veterinary Pathology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
Transbound Emerg Dis. 2019 Jan;66(1):381-388. doi: 10.1111/tbed.13032. Epub 2018 Oct 16.
Mucohaemorrhagic diarrhea associated with Brachyspira hampsonii infection has emerged as a production-limiting disease in western Canada. This pathogen was first described in North America in 2010, and reports of its detection occurred concurrently in western Canada and the United States. Since that time, Brachyspira hampsonii has been detected in Europe, both in pigs and in waterfowl. The origin of B. hampsonii and the timing and reasons for its emergence are unknown. We conducted a retrospective study of historic, archived cases of porcine colitis to determine when B. hampsonii was first evident in western Canada. A total of 206 samples from 114 cases submitted from 57 different farms or productions systems in 1984 and 1999-2009 were screened using real-time PCR assays targeting B. hampsonii genomovars I and II, and Brachyspira hyodysenteriae (the traditional agent of swine dysentery). In most cases, positive real-time PCR results were confirmed by amplification and sequencing of additional gene targets. A total of 9, 7 and 5 samples tested positive for B. hampsonii (I), B. hampsonii (II) or B. hyodysenteriae respectively. The results of this study push the date of first appearance of B. hampsonii in pigs in western Canada back to 2002 (B. hampsonii (I)) and 2006 (B. hampsonii (II)), which is up to 7 years before the new species were first identified in fresh samples.
与伯氏疏螺旋体感染相关的黏液血性腹泻已成为加拿大西部限制生产的疾病。该病原体于 2010 年首次在北美被描述，有关其检测的报告同时发生在加拿大西部和美国。自那时以来，伯氏疏螺旋体已在欧洲的猪和水禽中被检测到。伯氏疏螺旋体的起源以及其出现的时间和原因尚不清楚。我们对历史上存档的猪结肠炎病例进行了回顾性研究，以确定伯氏疏螺旋体何时首次出现在加拿大西部。使用针对伯氏疏螺旋体基因组型 I 和 II 以及猪痢疾密螺旋体（传统的猪痢疾病原体）的实时 PCR 检测方法，对来自 1984 年和 1999-2009 年来自 57 个不同农场或生产系统的 114 个病例的 206 个样本进行了筛选。在大多数情况下，通过扩增和测序其他基因靶标确认了阳性实时 PCR 结果。共有 9、7 和 5 个样本分别检测到伯氏疏螺旋体（I）、伯氏疏螺旋体（II）或猪痢疾密螺旋体阳性。本研究的结果将伯氏疏螺旋体在加拿大西部猪中的首次出现日期推回到 2002 年（伯氏疏螺旋体（I））和 2006 年（伯氏疏螺旋体（II）），比该新物种在新鲜样本中首次被鉴定早了长达 7 年。
