Bylund D B
Anal Biochem. 1986 Nov 15;159(1):50-7. doi: 10.1016/0003-2697(86)90306-4.
One technique for the characterization of receptor subtypes involves measuring the inhibition of the binding of a radioligand which is not subtype selective by agonists or antagonists which are subtype selective. Although such data are routinely calculated using computer programs, it is often useful to have a graphical representation of the data. Until now, a "modified Scatchard" or "Hofstee" plot of the form P = -(P/I)(IC50) + 1 (where P is percentage inhibition and I is the inhibitor concentration) has been used. We describe an alternate plot of the form B = -(B X I)(1/IC50) + B0 (where B is the concentration of bound radioligand and B0 is the concentration of radioligand bound in the absence of the inhibitor). This method has the important advantage that the data need not be calculated as percentage inhibition which eliminates the error involved in the experimentally determined B0 value being included in the other values.
一种用于表征受体亚型的技术涉及测量非亚型选择性放射性配体与亚型选择性激动剂或拮抗剂结合的抑制作用。尽管此类数据通常使用计算机程序进行计算，但以图形方式表示数据往往很有用。到目前为止，一直使用形式为P = -(P/I)(IC50) + 1的“修正Scatchard”或“Hofstee”图（其中P为抑制百分比，I为抑制剂浓度）。我们描述了一种形式为B = -(B×I)(1/IC50) + B0的替代图（其中B为结合放射性配体的浓度，B0为在无抑制剂情况下结合的放射性配体浓度）。该方法具有重要优势，即数据无需计算为抑制百分比，从而消除了实验测定的B0值包含在其他值中所涉及的误差。
