Tsunedomi Akari, Miyawaki Katsuyuki, Masamura Akinori, Nakahashi Mutsumi, Mawatari Kazuaki, Shimohata Takaaki, Uebanso Takashi, Kinouchi Yousuke, Akutagawa Masatake, Emoto Takahiro, Takahashi Akira
Department of Preventive Environment and Nutrition, Graduate School of Nutritional Science, Tokushima University.
Department of Agroscience, Bioresource Science and Economics, Graduate School of Technology, Industrial and Social Sciences, University of Tokushima.
J Med Invest. 2018;65(3.4):171-176. doi: 10.2152/jmi.65.171.
The number of plant factories in which crops are cultivated in an artificial environment has been increasing every year. In cultivation techniques involving hydroponics, plants are supplied with a circulating nutrient solution, which can become contaminated by pathogens that can propagate and spread throughout plant factories. Therefore, strategies to disinfect hydroponic nutrient solutions are needed. In this study, we developed a new disinfection device equipped with an ultraviolet A (UVA) light emitting diode (LED) that can be used to disinfect hydroponic nutrient solutions in plant factories. We first evaluated the basic disinfection capability of the device and then estimated its bactericidal effect in a small scale model system. The log survival ratio was related to UVA irradiation fluence and the volume of nutrient solution. From the assay results, we devised a kinetics equation to describe the relationship between nutrient solution volume, log survival ratio, and UVA fluence. Together our results show that UVA irradiation could be used to disinfect hydroponic nutrient solutions, and the derived kinetics equations can be used to determine optimal conditions, such as nutrient solution volume, UVA irradiation, and killing activity, to develop devices that disinfect hydroponic nutrient solutions. J. Med. Invest. 65:171-176, August, 2018.
在人工环境中种植作物的植物工厂数量逐年增加。在涉及水培的栽培技术中，植物被供应循环营养液，而这种营养液可能会被病原体污染，这些病原体能够在植物工厂中繁殖和传播。因此，需要有水培营养液消毒策略。在本研究中，我们开发了一种配备紫外线A（UVA）发光二极管（LED）的新型消毒装置，可用于对植物工厂中的水培营养液进行消毒。我们首先评估了该装置的基本消毒能力，然后在小规模模型系统中估计了其杀菌效果。对数存活比率与UVA辐照通量和营养液体积有关。根据测定结果，我们设计了一个动力学方程来描述营养液体积、对数存活比率和UVA通量之间的关系。我们的结果共同表明，UVA辐照可用于消毒水培营养液，并且推导得到的动力学方程可用于确定最佳条件，如水培营养液体积、UVA辐照和杀菌活性，以开发用于消毒水培营养液的装置。《医学调查杂志》65:171 - 176，2018年8月。
