Wright K E, Rosenthal K L, Rawls W E
Arch Virol. 1987;92(3-4):197-209. doi: 10.1007/BF01317477.
Adult inbred MHA hamsters are susceptible to lethal infections with Pichinde virus while inbred LSH hamsters resist such infections. Previous studies demonstrated higher levels of endogenous and induced natural killer (NK) activity in MHA splenocytes than in LSH splenocytes. Preferential replication of Pichinde virus in cells with NK activity was suggested by showing that the greater numbers of infected spleen cells observed in MHA hamsters could be accounted for by a cell population that cosedimented with a peak of NK activity. Increased cellularity of thymi and spleens as well as increased cells sensitive to lymphokines was also found in MHA hamsters as compared to LSH hamsters. In the present study we found that injection of anti-asialo GM 1 serum reduced NK activity but did not alter susceptibility to virus infection. However, MHA hamsters were found to be relatively deficient in the production of interleukin 2 and injection of interleukin 2 altered the mortality of hamsters infected with Pichinde virus. These findings suggest that susceptibility to lethal infection by Pichinde virus is associated with reduced ability to produce interleukin 2 in MHA hamsters.
成年近交系MHA仓鼠对皮钦德病毒的致死性感染敏感，而近交系LSH仓鼠则能抵抗这种感染。先前的研究表明，MHA脾细胞中的内源性和诱导性自然杀伤（NK）活性水平高于LSH脾细胞。通过显示在MHA仓鼠中观察到的更多感染脾细胞可由与NK活性峰值共沉降的细胞群体来解释，提示皮钦德病毒在具有NK活性的细胞中优先复制。与LSH仓鼠相比，MHA仓鼠还发现胸腺和脾脏的细胞增多以及对淋巴因子敏感的细胞增多。在本研究中，我们发现注射抗唾液酸GM1血清可降低NK活性，但不会改变对病毒感染的易感性。然而，发现MHA仓鼠在白细胞介素2的产生方面相对不足，注射白细胞介素2可改变感染皮钦德病毒的仓鼠的死亡率。这些发现表明，MHA仓鼠对皮钦德病毒致死性感染的易感性与产生白细胞介素2的能力降低有关。
