Miyasaka N, Darnell B, Baron S, Talal N
Cell Immunol. 1984 Mar;84(1):154-62. doi: 10.1016/0008-8749(84)90086-8.
Purified Interleukin 2 (IL-2), free of interferon (IFN), significantly enhanced NK activity of normal human peripheral blood mononuclear cells (PBMC). This enhancing activity was absorbed by IL-2 receptor-bearing cells but was not blocked by antibody to alpha-IFN. IFN in the culture supernatants was greatly increased after stimulation with poly(I:C) plus IL-2. There was less IFN produced by either modulator acting alone. Stimulation of PBMC with IL-2 and/or poly(I:C) increased the proportion of OKM1+ cells and anti-Leu-7+ cells. When cells expressing either surface antigen were specifically lysed to deplete NK, cytotoxic activity could be restored by overnight incubation in IL-2. This result suggests that IL-2 stimulates the development of NK cells from precursors that lack cell surface OKM1 or Leu-7. IL-2 acted directly on large granular lymphocytes and did not require the presence of adherent cells. These results suggest that IL-2 may act synergistically with other IFN inducers and may play an important role in the regulation of NK cells.
纯化的不含干扰素（IFN）的白细胞介素2（IL-2）能显著增强正常人外周血单个核细胞（PBMC）的自然杀伤（NK）活性。这种增强活性被携带IL-2受体的细胞吸收，但不被α-干扰素抗体阻断。用聚肌苷酸-聚胞苷酸（poly(I:C)）加IL-2刺激后，培养上清液中的IFN大幅增加。单独使用任何一种调节剂产生的IFN较少。用IL-2和/或poly(I:C)刺激PBMC可增加OKM1+细胞和抗Leu-7+细胞的比例。当表达任一表面抗原的细胞被特异性裂解以耗尽NK时，通过在IL-2中过夜孵育可恢复细胞毒性活性。该结果表明，IL-2刺激缺乏细胞表面OKM1或Leu-7的前体细胞发育为NK细胞。IL-2直接作用于大颗粒淋巴细胞，不需要贴壁细胞的存在。这些结果表明，IL-2可能与其他IFN诱导剂协同作用，可能在NK细胞的调节中起重要作用。
