Chen Moutong, Cheng Jianheng, Wu Qingping, Zhang Jumei, Chen Yuetao, Xue Liang, Lei Tao, Zeng Haiyan, Wu Shi, Ye Qinghua, Bai Jianling, Wang Juan
State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangzhou, China.
College of Food Science, South China Agricultural University, Guangzhou, China.
Front Microbiol. 2018 Sep 19;9:2215. doi: 10.3389/fmicb.2018.02215. eCollection 2018.
is an important Gram-positive foodborne pathogen. However, limited information is available on the comprehensive investigation and potential risk of in fresh aquatic products, which are popular to consumers in China. This study aimed to determine the occurrence, virulence profiles, and population diversity of isolated from aquatic products in China. In total, 846 aquatic product samples were collected between July 2011 and April 2016 from 43 cities in China. Approximately 7.92% (67/846) aquatic product samples were positive for , 86.57% positive samples ranged from 0.3 to 10 MPN/g, whereas 5.97% showed over 110 MPN/g by the Most Probable Number method, which included two samples of products intended to be eaten raw. Serogroups I.1 (serotype 1/2a), I.2 (serotype 1/2b), and III (serotype 4c) were the predominant serogroups isolated, whereas serogroup II.1 (serotype 4b) was detected at much lower frequencies. Examination of antibacterial resistance showed that nine antibacterial resistance profiles were exhibited in 72 isolates, a high level susceptibility of 16 tested antibiotics against were observed, indicating these common antibacterial agents are still effective for treating infection. Multilocus sequence typing revealed that ST299, ST87, and ST8 are predominant in aquatic products, indicating that the rare ST299 (serotype 4c) may have a special ecological niche in aquatic products and associated environments. Except and , the 72 isolates harbor nine virulence genes (, and ), premature stop codons (PMSCs) in were found in four isolates, three of which belonged to ST9. A novel PMSC was found in 2929-1LM with a nonsense mutation at position 1605 (TGG→TGA). All ST87 isolates harbored the gene, whereas 8 isolates (11.11%) carried the gene, and mainly belonged to ST1, ST3, ST308, ST323, ST330, and ST619. Taken together, these results first reported potential virulent isolates (ST8 and ST87) were predominant in aquatic products which may have implications for public health in China. It is thus necessary to perform continuous surveillance for in aquatic products in China.
是一种重要的革兰氏阳性食源性病原体。然而，关于中国消费者喜爱的新鲜水产品中该菌的全面调查和潜在风险的信息有限。本研究旨在确定从中国水产品中分离出的该菌的发生率、毒力谱和种群多样性。2011年7月至2016年4月期间，共从中国43个城市采集了846份水产品样本。通过最大可能数法，约7.92%（67/846）的水产品样本对该菌呈阳性，86.57%的阳性样本范围为0.3至10 MPN/g，而5.97%的样本显示超过110 MPN/g，其中包括两份打算生食的产品样本。分离出的主要血清群为I.1（血清型1/2a）、I.2（血清型1/2b）和III（血清型4c），而II.1（血清型4b）血清群的检出频率低得多。抗菌药物耐药性检测表明，72株分离株呈现出9种抗菌药物耐药谱，观察到16种受试抗生素对该菌具有高度敏感性，表明这些常用抗菌药物对治疗该菌感染仍然有效。多位点序列分型显示，ST299、ST87和ST8在水产品中占主导地位，表明罕见的ST299（血清型4c）可能在水产品及其相关环境中有特殊的生态位。除了和，72株分离株携带9个毒力基因（、和），在4株分离株中发现了中的提前终止密码子（PMSCs），其中3株属于ST9。在2929-1LM中发现了一个新的PMSC，在第1605位有一个无义突变（TGG→TGA）。所有ST87分离株都携带基因，而8株（11.11%）携带基因，主要属于ST1、ST3、ST308、ST323、ST330和ST619。综上所述，这些结果首次报道了潜在的有毒力的分离株（ST8和ST87）在水产品中占主导地位，这可能对中国的公共卫生有影响。因此，有必要对中国水产品中的进行持续监测。
