Alam Muntasir, Islam Md Saiful, Jahan M Ishrat, Deb Arpita Shyama, Rahman Afruna, Islam Zahidul, Chowdhury Atique Iqbal, Islam Kazi Munisul, Hossain Mohammad Zahid, Ahmed Dilruba, Arifeen Shams El, Gurley Emily S, Rahman Mustafizur
International Centre for Diarrhoeal Disease Research, Infectious Diseases Division, 68 Shaheed Tajuddin Ahmed Sarani, (icddr,b), Dhaka, Mohakhali, 1212, Bangladesh.
Division of Genomics & Transcriptomics, Joint Research Center for Human Retrovirus Infection, Kumamoto University, Kumamoto, Japan.
BMC Microbiol. 2025 Feb 3;25(1):61. doi: 10.1186/s12866-024-03650-5.
Listeria monocytogenes is a foodborne pathogen that can lead to severe pregnancy outcomes. This study reports the clinical and genomic characteristics of a Listeria-mediated stillbirth identified in January 2022 through the Child Health and Mortality Prevention Surveillance (CHAMPS) project in Bangladesh. The Lm-BD-CHAMPS-01 isolate was recovered from the blood and cerebrospinal fluid (CSF) of a male stillborn. Maternal history, clinical, and demographic data were collected by the CHAMPS surveillance platform. An expert panel evaluated all reports to determine the role of L. monocytogenes infection in the causal chain of stillbirth. Genomic characterization included multilocus sequence typing (MLST), core genome MLST (cgMLST), serotyping, and the presence or absence of virulence genes. Genetic divergence and phylogenetic analyses were conducted to determine the relationship with other reported isolates globally.
The isolate Lm-BD-CHAMPS-01 was identified as a novel cgMLST CT11424. It belonged to ST 308, Serotype 4b, Clonal Complex 1, and Phylogenetic Lineage 1. Key L. monocytogenes virulence genes facilitating the crossing of the placental barrier, including full-length inlA, LIPI-1, and LIPI-3, were detected. The isolate was closely related to clinical L. monocytogenes isolates, as determined by GrapeTree based on cgMLST. SNP-based phylogenetic analysis found Lm-BD-CHAMPS-01 to be the most distant from other CC1 isolates in the database. Possible sources of infection included the consumption of contaminated raw vegetables or exposure to pigeons.
This is the first genome sequence of clinical L. monocytogenes from Bangladesh, which also caused stillbirth. Rural healthcare professionals should be aware of L. monocytogenes infection risks during pregnancy. Pregnant women should be counseled on the dangers of exposure to animals or birds and consumption of potentially contaminated raw food to prevent adverse pregnancy outcomes due to L. monocytogenes infection.
单核细胞增生李斯特菌是一种食源性病原体，可导致严重的妊娠结局。本研究报告了2022年1月通过孟加拉国儿童健康与死亡率预防监测（CHAMPS）项目确定的一例由李斯特菌介导的死产的临床和基因组特征。Lm-BD-CHAMPS-01分离株是从一名男性死产儿的血液和脑脊液（CSF）中分离出来的。CHAMPS监测平台收集了产妇病史、临床和人口统计学数据。一个专家小组评估了所有报告，以确定单核细胞增生李斯特菌感染在死产因果链中的作用。基因组特征分析包括多位点序列分型（MLST）、核心基因组MLST（cgMLST）、血清分型以及毒力基因的有无。进行了遗传差异和系统发育分析，以确定与全球其他报告的分离株之间的关系。
分离株Lm-BD-CHAMPS-01被鉴定为一种新型的cgMLST CT11424。它属于ST 308、血清型4b、克隆复合体1和系统发育谱系1。检测到促进胎盘屏障穿越的关键单核细胞增生李斯特菌毒力基因，包括全长inlA、LIPI-1和LIPI-3。根据基于cgMLST的GrapeTree分析，该分离株与临床单核细胞增生李斯特菌分离株密切相关。基于单核苷酸多态性（SNP）的系统发育分析发现，Lm-BD-CHAMPS-01与数据库中其他CC1分离株的亲缘关系最远。可能的感染源包括食用受污染的生蔬菜或接触鸽子。
这是来自孟加拉国的临床单核细胞增生李斯特菌的首个基因组序列，该菌株也导致了死产。农村医疗保健专业人员应意识到孕期单核细胞增生李斯特菌感染的风险。应向孕妇提供咨询，告知其接触动物或鸟类以及食用可能受污染的生食的危险，以预防因单核细胞增生李斯特菌感染导致的不良妊娠结局。
