Green F A, Herman C A, Herman R P, Claesson H E, Hamberg M
Biochem Biophys Res Commun. 1987 Jan 30;142(2):309-14. doi: 10.1016/0006-291x(87)90274-9.
The metabolites of inflammatory cells produced by massive infection of the peritoneal cavity of two related European species of frogs, Rana temporaria and Rana arvalis were examined for lipoxygenase-generated products of exogenous arachidonic acid. Cells of Rana temporaria produced large amounts of 5-hydroxyeicosatetraenoic acid and leukotriene B4. Cells from Rana arvalis produced only 15-hydroxyeicosatetraenoic acid. This is the first unequivocal demonstration of such enzyme activity in lower vertebrates. There was a trend towards increased mortality in the species without evidence of 5-lipoxygenase activity.
对欧洲两种相关蛙类，即林蛙和草蛙腹腔大量感染时炎症细胞产生的代谢产物进行了检测，以分析外源花生四烯酸经脂氧合酶生成的产物。林蛙细胞产生大量5-羟基二十碳四烯酸和白三烯B4。草蛙细胞仅产生15-羟基二十碳四烯酸。这是首次在低等脊椎动物中明确证实这种酶活性。在没有5-脂氧合酶活性证据的物种中，存在死亡率上升的趋势。
