Green F A
Department of Physiological Chemistry, Karolinska Institutet, Stockholm, Sweden.
Inflammation. 1988 Feb;12(1):45-50. doi: 10.1007/BF00915891.
Exogenous arachidonic acid was trapped in human leukocytes with ethanol at 0 degrees C and 16 degrees C. Leukotriene B4 and its isomers were produced from the trapped substrate, and this production continued for at least 24 h at 0 degrees C. Synthesis from trapped substrate of these eicosanoids increased as a function of the incubation temperature. The ability to study leukotriene synthesis and intercellular transfer from exogenous but intracellularly trapped substrate without the multiple effects of cell activators may be of value in elucidating the metabolism and regulatory properties of these lipoxygenase products.
在0摄氏度和16摄氏度下，用乙醇将外源性花生四烯酸捕获在人白细胞中。白三烯B4及其异构体由捕获的底物产生，并且这种产生在0摄氏度下至少持续24小时。这些类二十烷酸从捕获底物的合成随着孵育温度的升高而增加。在没有细胞激活剂多种效应的情况下，研究从外源性但细胞内捕获的底物合成白三烯以及细胞间转移的能力，可能有助于阐明这些脂氧合酶产物的代谢和调节特性。
