Institute of Human Genetics, Ulm University and Ulm University Medical Center, Ulm, Germany.
Institute of Human Genetics, Christian-Albrechts-University Kiel & University Hospital Schleswig-Holstein, Campus Kiel, Kiel, Germany.
Genes Chromosomes Cancer. 2019 Jan;58(1):66-69. doi: 10.1002/gcc.22687. Epub 2018 Nov 29.
Primary lymphomas of the central nervous system (PCNSL) are diffuse large B-cell lymphomas (DLBCLs) confined to the central nervous system (CNS). We here performed array-based DNA methylation analyses of 26 PCNSL and 78 DLBCL and validated our findings in an independent dataset. We identified 2847 CpGs differentially methylated between PCNSL and non-CNS-DLBCL. Neither a supervised analysis using these CpGs nor application of 3 CpG classifiers selected for class separation unambiguously separated PCNSL from non-CNS-DLBCL. Remarkably, 6/78 non-CNS-DLBCL consistently segregated with PCNSL, which displayed molecular features typical for PCNSL. Our findings suggest that a subset of non-CNS-DLBCL exists which molecularly resembles PCNSL.
原发性中枢神经系统淋巴瘤（PCNSL）是局限于中枢神经系统（CNS）的弥漫性大 B 细胞淋巴瘤（DLBCL）。我们在此对 26 例 PCNSL 和 78 例 DLBCL 进行了基于阵列的 DNA 甲基化分析，并在独立数据集进行了验证。我们在 PCNSL 和非 CNS-DLBCL 之间鉴定了 2847 个 CpG 差异甲基化。使用这些 CpG 进行的监督分析或选择用于分类分离的 3 个 CpG 分类器都不能明确地区分 PCNSL 和非 CNS-DLBCL。值得注意的是，6/78 例非 CNS-DLBCL 始终与 PCNSL 一致，PCNSL 表现出典型的 PCNSL 分子特征。我们的研究结果表明，存在一部分非 CNS-DLBCL，其分子特征类似于 PCNSL。
