College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.
Poult Sci. 2019 Mar 1;98(3):1111-1120. doi: 10.3382/ps/pey474.
In our previous study, chondrocyte apoptosis in femoral head necrosis (FHN)-affected broilers was found to be associated with the endoplasmic reticulum stress (ERS) signaling pathway. In the present study, we further explored the role of ERS-induced chondrocyte apoptosis in FHN-affected broilers and the parallel test was carried out with articular chondrocytes cultivated in vitro. The broilers and chondrocytes were treated with methylprednisolone (MP). The main pathological changes in FHN-affected broilers included the proximal femoral head separated from its articular cartilage and growth plate lesions. MP-treated chondrocytes demonstrated morphology changes, cell viability reduction, secretory capacity dysfunction, and apoptosis. The mRNA expressions of pro-apoptotic genes controlled by ERS signaling pathway were up-regulated both in vivo and in vitro experiments. It showed that MP induced FHN in broilers, activated apoptosis-related genes on ERS signaling pathway, and affected the survival and apoptosis of chondrocytes, and bone growth.
在我们之前的研究中，发现股骨头坏死（FHN）肉鸡的软骨细胞凋亡与内质网应激（ERS）信号通路有关。在本研究中，我们进一步探讨了 ERS 诱导的软骨细胞凋亡在 FHN 肉鸡中的作用，并进行了关节软骨细胞体外培养的平行试验。用甲泼尼龙（MP）处理肉鸡和软骨细胞。FHN 肉鸡的主要病理变化包括股骨头近端与关节软骨和生长板分离。MP 处理的软骨细胞表现出形态变化、细胞活力降低、分泌功能障碍和凋亡。体内和体外实验均显示 ERS 信号通路调控的促凋亡基因的 mRNA 表达上调。结果表明，MP 诱导了肉鸡的 FHN，激活了 ERS 信号通路相关的凋亡基因，并影响了软骨细胞的存活和凋亡以及骨骼生长。
