Li P F, Zhou Z L, Shi C Y, Hou J F
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China
Poult Sci. 2015 May;94(5):1052-9. doi: 10.3382/ps/pev071. Epub 2015 Mar 11.
Femoral head necrosis (FHN) is a metabolic cartilage disease of rapidly growing broilers. The aim of the present study was to investigate the role of basic fibroblast growth factor (bFGF) in the apoptotic processes associated with FHN. Broilers were selected and categorized based on clinical examination in 3 groups: healthy, femoral head separation, or femoral head separation with growth plate lacerations. Hematoxylin and eosin staining showed fewer chondrocytes in the resting zone of the growth plates when FHN occurred. Moreover, the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay revealed a significant increase in chondrocyte apoptosis. Furthermore, immunohistochemical assays and real-time quantitative PCR analysis demonstrated a decline in bFGF expression. In addition, reduced Bcl-2 mRNA expression was observed along with a corresponding increase in Bax and caspase-3 mRNA expression in FHN samples. There was a correlation between bFGF protein expression and the proportion of TUNEL-positive cells and a correlation between bFGF mRNA expression and expression of Bax, and caspase-3. The results of the study suggested that the expression of bFGF was reduced in the process of chondrocyte apoptosis, which could play an important role in the pathogenesis of FHN in chickens.
股骨头坏死（FHN）是快速生长的肉鸡的一种代谢性软骨疾病。本研究的目的是探讨碱性成纤维细胞生长因子（bFGF）在与FHN相关的凋亡过程中的作用。根据临床检查将肉鸡分为3组：健康组、股骨头分离组或伴有生长板撕裂的股骨头分离组。苏木精-伊红染色显示，FHN发生时，生长板静止区的软骨细胞减少。此外，末端脱氧核苷酸转移酶dUTP缺口末端标记（TUNEL）检测显示软骨细胞凋亡显著增加。此外，免疫组织化学检测和实时定量PCR分析表明bFGF表达下降。此外，在FHN样本中观察到Bcl-2 mRNA表达降低，同时Bax和caspase-3 mRNA表达相应增加。bFGF蛋白表达与TUNEL阳性细胞比例之间存在相关性，bFGF mRNA表达与Bax和caspase-3表达之间存在相关性。研究结果表明，bFGF在软骨细胞凋亡过程中表达降低，这可能在鸡FHN的发病机制中起重要作用。
