United States Department of Agriculture, Agricultural Research Service, Harry K. Dupree Stuttgart National Aquaculture Research Center, 2955 Hwy. 130 East, Stuttgart, AR, 72160, USA.
FEMS Microbiol Lett. 2018 Nov 1;365(22). doi: 10.1093/femsle/fny244.
Columnaris disease, caused by Flavobacterium columnare, severely impacts the production of freshwater finfish species. Therefore, efforts to better understand the biological processes of F. columnare, including the formation of biofilms and their contribution to disease, are ongoing. In this study, we incubated F. columnare cultures with channel catfish mucus and used high-throughput RNA sequencing to evaluate global changes in gene expression. Our data show that mucus activates in vitro biofilm formation. The analysis of F. columnare transcriptomes after the addition of mucus revealed significant differentially expressed genes (DEGs) between the planktonic and biofilm states. DEGs common among all biofilms were enriched for gene ontology groups including signal transduction, ligand binding and cellular homeostasis and are likely necessary for biofilm formation. Iron acquisition systems included TonB-dependent receptor and ferroxidase genes were expressed among all biofilms, while siderophore synthesis genes were only expressed in mucus-stimulated biofilms. The current analysis of F. columnare transcriptomes adds valuable information about the basic biological processes that occur during the planktonic and biofilm states. This work serves as a basis for future studies on understanding how biofilms are established and how they contribute to disease progression.
柱状屈挠杆菌病由柱状屈挠杆菌引起，严重影响淡水养殖鱼类的生产。因此，人们正在努力更好地了解柱状屈挠杆菌的生物学过程，包括生物膜的形成及其对疾病的贡献。在这项研究中，我们用斑点叉尾鮰黏液孵育柱状屈挠杆菌培养物，并使用高通量 RNA 测序来评估基因表达的全局变化。我们的数据表明，黏液能激活体外生物膜的形成。在添加黏液后分析柱状屈挠杆菌的转录组，发现浮游和生物膜状态之间存在显著差异表达基因（DEGs）。所有生物膜中共有的 DEGs 富集了信号转导、配体结合和细胞内稳态等基因本体组，这些基因可能是生物膜形成所必需的。铁获取系统包括 TonB 依赖性受体和亚铁氧化酶基因在所有生物膜中表达，而铁载体合成基因仅在黏液刺激的生物膜中表达。目前对柱状屈挠杆菌转录组的分析增加了有关浮游和生物膜状态下发生的基本生物学过程的有价值信息。这项工作为未来研究理解生物膜的建立以及它们如何促进疾病进展奠定了基础。
