Koike T, Aoki S, Maruyama S, Narita M, Ishizuka T, Imanaka H, Adachi T, Maeda H, Shibata A
Blood. 1987 Mar;69(3):957-60.
Surface phenotypic characterization of megakaryoblasts, identified by platelet peroxidase activity, was investigated in four patients who showed increased proliferation of megakaryoblasts: one patient with typical features of acute leukemia, one presenting with acute myelofibrosis, and two with Down's syndrome in whom blasts disappeared spontaneously (transient abnormal myelopoiesis, TAM). MY10 and/or MY9 antigens were expressed on the surface of some megakaryoblasts, but MY7, and MY4, antigens specific to granulocytic or monocytic cells, were not. Some megakaryoblasts were positive for only anti-HLA-DR antibodies. It was speculated that, during the differentiation of the megakaryocytic lineage, MY9 antigen appears transiently on the surface of megakaryoblasts that have lost HLA-DR antigens and have gained the glycoprotein IIb/IIIa antigen. This study also demonstrated that the proliferating blasts in some patients with TAM were mainly megakaryoblasts and suggested that the target cells in TAM are CFU-GEMM.
通过血小板过氧化物酶活性鉴定的巨核母细胞的表面表型特征，在4例巨核母细胞增殖增加的患者中进行了研究：1例具有典型急性白血病特征，1例表现为急性骨髓纤维化，2例唐氏综合征患者，其原始细胞自发消失（短暂异常髓系造血，TAM）。一些巨核母细胞表面表达MY10和/或MY9抗原，但粒细胞或单核细胞特异性的MY7和MY4抗原未表达。一些巨核母细胞仅对抗HLA-DR抗体呈阳性。据推测，在巨核细胞系分化过程中，MY9抗原短暂出现在已失去HLA-DR抗原并获得糖蛋白IIb/IIIa抗原的巨核母细胞表面。本研究还表明，一些TAM患者中增殖的原始细胞主要是巨核母细胞，并提示TAM中的靶细胞是CFU-GEMM。
